Single-cell Raman and functional gene analyses reveal microbial P solubilization in agriculture waste-modified soils  

作　　者：Hongzhe Li Jiazhi Ding Longji Zhu Fei Xu Wenjing Li Yanpo Yao Li Cui 

机构地区：[1]Key Lab of Urban Environment and Health,Institute of Urban Environment,Chinese Academy of Sciences,Xiamen,China [2]College of Life Sciences,Fujian Agriculture and Forestry University,Fuzhou,China [3]University of Chinese Academy of Sciences,Beijing,China [4]Agro‐Environmental Protection Institute,Ministry of Agriculture and Rural Affairs,Tianjin,China

出　　处：《mLife》2023年第2期190-200,共11页微生物（英文）

基　　金：funded by the National Natural Science Foundation of China(42021005,22241603);the Chinese Academy of Sciences(ZDBS‐LY‐DQC027).

摘　　要：Application of agricultural waste such as rapeseed meal(RM)is regarded as a sustainable way to improve soil phosphorus(P)availability by direct nutrient supply and stimulation of native phosphate‐solubilizing microorganisms(PSMs)in soils.However,exploration of the in situ microbial P solubilizing function in soils remains a challenge.Here,by applying both phenotype‐based single‐cell Raman with D_(2)O labeling(Raman‐D_(2)O)and genotype‐based high‐throughput chips targeting carbon,nitrogen and P(CNP)functional genes,the effect of RM application on microbial P solubilization in three typical farmland soils was investigated.The abundances of PSMs increased in two alkaline soils after RM application identified by single‐cell Raman D_(2)O.RM application reduced the diversity of bacterial communities and increased the abundance of a few bacteria with reported P solubilization function.Genotypic analysis indicated that RM addition generally increased the relative abundance of CNP functional genes.A correlation analysis of the abundance of active PSMs with the abundance of soil microbes or functional genes was carried out to decipher the linkage between the phenotype and genotype of PSMs.Myxococcota and C degradation genes were found to potentially contribute to the enhanced microbial P release following RM application.This work provides important new insights into the in situ function of soil PSMs.It will lead to better harnessing of agricultural waste to mobilize soil legacy P and mitigate the P crisis.

关 键 词：CNP functional genes D_(2)O isotope labeling phosphate-solubilizing microorganisms single-cell Raman 

分 类 号：S154.3[农业科学—土壤学]