传染性脾肾坏死病毒灭活疫苗对鳜肠道微生物的影响研究  

Effects of Inactivated Vaccine of Infectious Spleen and Kidney Necrosis Virus on Intestinal Microflora of Mandarin Fish Siniperca chuatsi

作　　者：何润真 朱凝瑜 梁倩蓉 郑晓叶 陈小明 姚高华 丁雪燕 HE Runzhen;ZHU Ningyu;LIANG Qianrong;ZHENG Xiaoye;CHEN Xiaoming;YAO Gaohua;DING Xueyan(Zhejiang Province Fisheries Technical Extension Center,Hangzhou 310023,China)

机构地区：[1]浙江省水产技术推广总站,浙江杭州310023

出　　处：《水产科学》2023年第5期862-870,共9页Fisheries Science

基　　金：国家特色淡水鱼产业技术体系项目(CARS-46);浙江省科技厅公益技术研究计划资助项目(LGN20C190010);浙江省“十四五”水产新品种选育重大科技专项(2021C02069-2)。

摘　　要：为探究接种传染性脾肾坏死病毒灭活疫苗对鳜肠道微生物的组成结构及相关功能基因的影响,对体质量(20±1.8) g的鳜进行腹腔注射传染性脾肾坏死病毒灭活疫苗,对照组注射等体积无菌生理盐水。对照组和免疫组放入用纱网隔开的同一池塘中养殖,28 d后每组各采集10尾肠道粪便样本,用高通量测序,构建文库。高通量测序共获得9669条序列,平均长度为543 bp。Alpha多样性分析结果显示,Chao1指数在对照组中更高,香农指数和辛普森指数在免疫组中更高,表明免疫后肠道微生物的多样性和丰度增加。在门水平上,对照组肠道微生物以变形菌门为优势菌门,相对丰度为72.23%;免疫组以变形菌门和衣原体门为优势菌门,相对丰度分别为28.57%和11.13%。在属水平上,对照组以气单胞菌属为主要优势属,相对丰度高达71.73%;免疫组以衣原体属和埃希氏杆菌属为主要优势属,相对丰度分别为11.57%和8.7%。免疫组与对照组相比,共筛选到9656个差异丰度基因,其中有840个基因高丰度,8816个基因低丰度。差异基因主要富集在双组分系统、ABC转运子、嘌呤代谢、群体感应、细菌趋药性、鞭毛组装和细菌分泌系统等通路,通路中的绝大部分基因的丰度在免疫组中均降低。上述结果表明,传染性脾肾坏死病毒灭活疫苗免疫后鳜肠道微生物的组成和功能基因变化明显,可引起致病菌维氏气单胞菌和嗜水气单胞菌比例的减少,同时引起乳酸菌属、芽孢杆菌属和类芽孢杆菌属等有益细菌比例增加,这可为了解疫苗的作用机制提供新思路。In order to explore the effect of Inactivated infectious spleen and kidney necrosis virus (ISKNV) vaccine on the composition, structure and related functional genes of gut microbes in mandarin fish Siniperca chuatsi , the mandarin fish with body weight of (20.0±1.8) g was intraperitoneally injected with the inactivated ISKNV vaccine, and was injected with an equal volume of sterile saline in the control group. The fish in the control group and the immunized group were reared in the same pond separated by an enclosure for 28 days when 10 intestinal fecal samples were collected from each group, and sequenced by high-throughput sequencing to construct a library. A total of 9669 Unigenes with an average length of 543 bp were obtained by high-throughput sequencing. Alpha diversity analysis showed that Chao1 index was higher in the control group, and Shannon index and Simpson index were higher in the immunized group, indicating that the variety was decreased and the diversity and richness were shown to be increased in gut microbes of the immunized mandarin fish. Proteobacteria was the dominant phyla in the control group, with abundance of 72.23%, at the phylum level, with the relative abundance of 28.57% in Proteobacteria and 11.13% in Chlamydia in the immune group. At the genus level, Aeromonas was the dominant genera in the control group, with the relative abundance of 71.73%. In the immunized group, Chlamydia and Escherichia were the dominant genera, with the abundance of 11.57% and 8.7%, respectively. Compared with the control group, 9656 differentially genes were enriched in the immunized group, with 840 high abundance genes were and 8816 low abundance genes. Differential genes were mainly enriched in the two-component system, ABC transporter, purine metabolism, quorum sensing, bacterial chemotaxis, flagellar assembly and bacterial secretion system, and the abundance of most genes in the pathway were decreased in the immunized group. Injection administration of inactivated ISKNV vaccine resulted in changes in int

关 键 词：鳜 传染性脾肾坏死病毒 灭活疫苗 肠道微生物 宏基因组 

分 类 号：S941.8[农业科学—水产养殖]