鸡传染性支气管炎病毒特异性单克隆抗体的制备与鉴定  

Preparation and Identification of Monoclonal Antibody against N Protein of Avian Infectious Bronchitis Virus

作　　者：薛麒[1] 侯力丹[1] 黄小洁[1] 秦义娴 毛娅卿[1] 孔冬妮[1] 王嘉[1] 吴华伟[1] 刘丹[1] XUE Qi;HOU Li-dan;HUANG Xiao-jie;QIN Yi-xian;MAO Ya-qing;KONG Dong-ni;WANG Jia;WU Hua-wei;LIU Dan(China Institute of Veterinary Drug Control,Beijing 100081,China)

机构地区：[1]中国兽医药品监察所,北京100081

出　　处：《中国兽药杂志》2023年第9期1-6,共6页Chinese Journal of Veterinary Drug

基　　金：公益性专项--禽源制品外源病毒检验新方法及标准物质的研究(GY202105)

摘　　要：为获得鸡传染性支气管炎病毒(Avian infectious bronchitis virus,IBV)N蛋白的单克隆抗体,通过原核表达IBVN蛋白,纯化后作为免疫原免疫BALB/c小鼠,并按常规方法制备杂交瘤细胞。经ELISA方法筛选阳性杂交瘤细胞,经过3次亚克隆获得3株杂交瘤细胞株,命名为1#、18#、19#,并进行了抗体亚类的鉴定、Western-blot和IFA检测。结果显示:制备的3株单克隆抗体亚型均为IgG1,Western-blot和IFA试验结果表明单克隆抗体均能与IBV发生特异性反应而与其他禽病常见病毒均无交叉反应。本研究成功制备了IBV单克隆抗体,为进一步建立IBV检测方法和深入研究IBV的生物学特性奠定了基础。In order to obtain the monoclonal antibody against N protein of Avian infectious bronchitis virus,the N protein of IBV was expressed in prokaryotic cells,purified and used as immunogen to immunize BALB/c mice,and the hybridoma cells were prepared according to the conventional method.Three hybridoma cell lines,named 1#,18#and 19#were obtained by ELISA.The antibody subclasses were identified,Western-blot and IFA were detected.The results showed that the three monoclonal antibodies were IgG1 subtype.Western-blot and IFA results showed that the monoclonal antibodies could specifically react with IBV and had no cross-reaction with other common avian viruses.In conclusion,the monoclonal antibody against N Protein of IBV was successfully prepared,which laid a foundation for further establishment of IBV detection methods and in-depth study of the biological characteristics of IBV.

关 键 词：鸡传染性支气管病毒 N蛋白 单克隆抗体 

分 类 号：S858.3[农业科学—临床兽医学]