超高效液相色谱-串联质谱法测定鸡蛋中沙拉沙星残留的研究  

Determination of Salafloxacin Residues in Eggs by Ultra Performance Liquid Chromatography-tandem Mass Spectrometry

作　　者：傅凤 孟蕾 杨洁 吴宁鹏 张宁 韩立 刘婷婷 吴志明 FU Feng;MENG Lei;YANG Jie;WU Ning-Peng;ZHANG Ning;HAN Li;LIU Ting-ting;WU Zhi-ming(College of Veterinary Medicine,Henan Agricultural University,Zhengzhou 450002,China;Henan Provincial Institute of Veterinary Drug and Feed Supervision,Zhengzhou 450008,China)

机构地区：[1]河南农业大学动物医学院,郑州450002 [2]河南省兽药饲料监察所,郑州450008

出　　处：《中国兽药杂志》2023年第9期28-35,共8页Chinese Journal of Veterinary Drug

摘　　要：建立了鸡蛋中沙拉沙星残留检测的液相色谱-串联质谱法。样品经1 mol/L磷酸和乙腈提取后,饱和正己烷除脂净化,以0.1%甲酸溶液和乙腈为流动相,液相色谱-串联质谱法测定,内标法定量。试验结果表明:沙拉沙星在1~25 ng/mL浓度范围内线性关系良好,相关系数r大于0.999。沙拉沙星在鸡全蛋及其蛋清、蛋黄中的检测限均为0.5μg/kg,定量限为1μg/kg。沙拉沙星在1、10、20μg/kg浓度添加水平内的平均回收率范围为97.0%~105.4%,批内、批间相对标准偏差在1.3%~10.3%范围之间。本方法适用于鸡全蛋及其蛋清、蛋黄中沙拉沙星残留量的测定。A liquid chromatography-tandem mass spectrometry(LC-MS)method was developed for the determination of salafloxacin residues in eggs.The sample was extracted with 1 mol/L phosphoric acid and acetonitrile,purified by saturated hexane,and determined by liquid chromatography-tandem mass spectrometry with 0.1%formic acid solution and acetonitrile as mobile phases,and quantified by internal standard method.The test results showed that the linearity of salafloxacin was good in the concentration range of 1~25 ng/mL,and the correlation coefficient r>0.999.The limits of detection were 0.5μg/kg and the limits of quantification were 1μg/kg for salafloxacin in whole egs,albumens and yolks.The average recoveries of salafloxacin at 1μg/kg,10μg/kg and 20μg/kg spiked levels ranged from 97.0%to 105.4%with the relative standard deviations(RSDs)of 1.3%-10.3%within and between batches.This method is suitable for the determination of salafloxacin residues in whole eggs,albumens and yolks.

关 键 词：沙拉沙星 鸡蛋 残留检测 液相色谱串联质谱法 

分 类 号：S859.84[农业科学—临床兽医学]