小麦粒重关键数量性状位点qTkw-2D遗传效应分析  

The Genetic Effect Analysis of the Major QTL qTkw-2D for Grain Weight in Wheat(Triticum aestivum)

作　　者：张娜 张帅 支丽雅 宋利强[2] 刘东涛[1] 马红勃[1] 纪军[2] ZHANG Na;ZHANG Shuai;ZHI Li-Ya;SONG Li-Qiang;LIU Dong-Tao;MAHong-Bo;JI Jun(Jiangsu Xuhuai Regional Institute of Agricultural Sciences,Xuzhou 221131,China;Center for Agricultural Resources Research,Institute of Genetics and Developmental Biology,Chinese Academy of Sciences,Shijiazhuang 050021,China)

机构地区：[1]江苏徐淮地区徐州农业科学研究所,徐州221131 [2]中国科学院遗传与发育生物学研究所农业资源研究中心,石家庄050021

出　　处：《农业生物技术学报》2023年第10期1999-2006,共8页Journal of Agricultural Biotechnology

基　　金：江苏省双创博士(JSSCBS20221814);徐州市农业科学院科研基金项目(RC2021001);山东省重点研发计划(2022LZGCQY022)。

摘　　要：小麦(Triticum aestivum)粒重遗传力高、表型稳定,高产育种过程中对粒重的选择效果显著。本研究在前期基因定位的基础上,获得了多环境稳定粒重QTL-qTkw-2D,且该位点在多个群体中被报道。本研究利用与qTkw-2D紧密连锁的分子标记Xcfd233对3000份育种高代系进行筛选,结合Wheat660K SNP芯片分型,在’科农2011’的F_(6)代获得了qTkw-2D近等基因系。通过对近等基因系籽粒切片的皮层细胞大小分析,明确了qTkw-2D主要通过影响皮层细胞大小影响粒宽。灌浆速率分析结果显示,q Tkw-2D主要通过影响中后期灌浆速率影响粒重。对近等基因系多年多地单株及小区水平的粒重和产量进行分析,明确了qTkw-2D位点A单倍型可稳定增加粒重,且在歉收环境对小麦稳产具有重要意义。本研究为小麦粒重遗传改良和粒重功能基因挖掘提供了参考。In wheat(Triticum aestivum),grain weight selecting is effective for improvement of grain yield as its high heredity and stable phenotype.In previous study,a stable QTL for grain weight,qTkw-2D,was identified and this QTL has been verified in several populations.In this study,the linkage marker Xcfd233 and Wheat660K SNP array were used to scan 3000 high generation of breeding population.Finally,near isogenic lines(NILs)of qTkw-2D were achieved in the F_(6) generation of'Kenong 2011'.By comparison the pericarp cells size of the grain slices from two NILs,it was confirmed that qTkw-2D mainly affected grain width by affecting the size of inner pericarp cells.The analysis results of filling rate showed that qTkw-2D mainly affected grain weight by affecting the grain filling rate during middle and late filling stage.The grain weight in different environments at individual and population level of NILs indicated that qTkw-2D influenced grain yield in both levels and haplotype A was of great importance to maintain grain yield during poor harvest environments.This study provides reference for grain weight improvement and function gene cloning.

关 键 词：粒重 QTL 近等基因系 遗传效应 

分 类 号：S512.1[农业科学—作物学]