江西地方鸡品种线粒体DNA ND6基因的遗传多样性  

Genetic Diversity of mtDNA ND6 gene in Jiangxi Chicken Breeds(Gallus gallus domesticus)

作　　者：贾晓旭[1] 陈皖强 唐修君[1] 樊艳凤[1] 陆俊贤[1] 马尹鹏 黄胜海[1] 葛庆联[1] 顾荣[1] 高玉时[1] JIA Xiao-Xu;CHEN Wan-Qiang;TANG Xiu-Jun;FAN Yan-Feng;LU Jun-Xian;MA Yin-Peng;HUANG Sheng-Hai;GE Qing-Lian;GU Rong;GAO Yu-Shi(Jiangsu Institute of Poultry Science,Yangzhou 225125,China)

机构地区：[1]江苏省家禽科学研究所,扬州225125

出　　处：《农业生物技术学报》2023年第10期2132-2139,共8页Journal of Agricultural Biotechnology

基　　金：国家重点研发计划(2021YFD1200302,2021YFD1200305);江苏省自然科学基金(BK20221412);江苏现代农业产业技术体系建设专项资金(JATS[2021]399);国家自然科学基金(31501917,31672382,31702079,31372277)。

摘　　要：江西省拥有众多的地方鸡(Gallus gallus domesticus)品种。为研究江西地方鸡品种的遗传多样性和系统发育关系,本研究采集了江西不同地区的6个地方鸡品种(安义瓦灰鸡,白耳黄鸡,崇仁麻鸡,丝羽乌骨鸡,东乡绿壳蛋鸡和余干乌骨鸡)的198份血液样本,采用PCR扩增与核酸测序技术相结合的方法对这6个江西地方鸡品种线粒体DNA(mitochondrial DNA,mtDNA)的还原型烟酰胺腺嘌呤二核苷酸(nicotinamide adenine dinucleotide,NADH)脱氢酶亚基6(NADH dehydrogenase subunit 6,ND6)基因全序列进行了检测。结果表明,6个江西地方鸡品种的ND6基因序列全长为522 bp,共计检测到7个核苷酸多态位点,根据序列变异共确定了7种单倍型,其中Hap1为主流单倍型。核苷酸多样性、单倍型多样性和平均核苷酸差异分别为0.00275±0.00086、0.694±0.024和1.437。中介网络图分析显示,6个江西地方鸡品种单倍型呈星状发散分布。系统发育分析发现,丝羽乌骨鸡、东乡绿壳蛋鸡和余干乌骨鸡聚为一支,安义瓦灰鸡、白耳黄鸡和崇仁麻鸡聚为一支。以上结果表明,江西地方鸡线粒体ND6基因多态信息含量丰富,乌骨鸡可能有不同的母系来源。该研究结果为江西地方鸡遗传资源的种质保护、品种选育和鉴定工作提供了参考。The Jiangxi province has numerous native domestic chicken breeds.This research aimed to characterize the genetic diversity and phylogeny of Jiangxi chicken(Gallus gallus domesticus)breeds.A total of 198 blood samples were collected from 6 breeds(Anyi Gray,Baier Yellow,Chongren Partridge,Silkie,Dongxiang Blue-eggshell,and Yugan Black-bone),reared in different areas of Jiangxi province.In this study,the PCR amplification method combined with nucleic acid sequencing technology was employed to detect the complete mitochondrial DNA(mtDNA)nicotinamide adenine dinucleotide(NADH)dehydrogenase subunit 6(ND6)sequences.The results demonstrated that the full length of ND6 gene of 6 Jiangxi chicken breeds was 522 bp.A total of 7 variable sites were identified,which defined 7 haplotypes,Hap1 was the mainstream haplotype.The differences in nucleotide diversity,haplotype diversity,and the average number of nucleotide differences were 0.00275±0.00086,0.694±0.024,and 1.437,respectively.The median network diagram exhibited that the haplotype of 6 Jiangxi chicken breeds presented a star-shaped divergent distribution.Phylogenetic analysis showed that Silkie,Dongxiang Blue-eggshell and Yugan Black-bone clustered into one branch,and Anyi Gray,Baier Yellow,and Chongren Partridge clustered into another branch.The mitochondrial ND6 gene polymorphism information of Jiangxi chicken breeds was abundant,black bone chickens may have a different maternal origin.The results of this study will provide a reference for the preservation,improvement,and identification of Jiangxi chicken breeds.

关 键 词：线粒体DNA(mtDNA) 遗传多样性 鸡 江西 系统发育 

分 类 号：S831.2[农业科学—畜牧学]