鸭circ_MBNL1在脂肪细胞分化过程中的功能预测  

Functional Prediction of circ_MBNL1 in Duck(Anas platyrhynchos domesticus)Adipocyte Differentiation

作　　者：张傲 王来娣[1] 杨婷 江勇 王志秀 陈国宏[1,2] 白皓 常国斌[1,2] ZHANG Ao;WANG Lai-Di;YANG Ting;JIANG Yong;WANG Zhi-Xiu;CHEN Guo-Hong;BAI Hao;CHANG Guo-Bin(Key Laboratory of Animal Genetic Breeding and Molecular Design in Jiangsu Province/College of Animal Science and Technology,Yangzhou University,Yangzhou 225009,China;Joint International Research Laboratory of Agriculture and Agri-Product Safety,Ministry of Education/Institute of Agricultural Science and Technology Development,Yangzhou University,Yangzhou 225009,China)

机构地区：[1]江苏省动物遗传育种与分子设计重点实验室/扬州大学动物科学与技术学院,扬州225009 [2]教育部农业与农产品安全国际合作联合实验室/扬州大学农业科技发展研究院,扬州225009

出　　处：《农业生物技术学报》2023年第10期2140-2149,共10页Journal of Agricultural Biotechnology

基　　金：国家水禽产业技术体系(CARS-42);江苏省水禽产业技术体系(JATS[2022]331)。

摘　　要：环状RNA(circular RNAs,circRNAs)是一种新型内源性非编码RNA,在各种组织中均有表达。本研究以樱桃谷鸭(Anas platyrhynchos domesticus)胚原代细胞分化过程中circRNA测序结果为基础,针对由肌肉盲样剪接调节因子1(musblind-like 1,MBNL1)基因编码的circ_MBNL1进行鉴定与功能分析。通过PCR扩增和焦磷酸测序技术获得接合序列,采用核质分离消化实验验证了鸭circ_MBNL1主要定位在细胞核中。组织表达谱结果提示,circ_MBNL1主要在鸭脾脏、脂肪和肺组织中表达。生物信息学分析结果表明,circ_MBNL1与预测的靶基因主要富集在脂肪细胞因子信号通路、FoxO信号等通路,推测circ_MBNL1可能参与调控鸭脂肪细胞的分化过程。本研究为深入探究circ_MBNL1在鸭脂肪细胞分化过程中的调控作用提供了理论基础。Circular RNAs(circRNAs),a novel endogenous non-coding RNA expressed in various tissues.In this paper,circ_MBNL1 encoded by MBNL1(musblind-like 1)gene was selected for identification and functional analyses based on the results of circRNA sequencing during the differentiation of duck(Anas platyrhynchos domesticus)embryo primary cells.The conjugated sequence was obtained by PCR amplification and pyrosequencing.And the nucleoplasmic separation assay revealed that duck circ_MBNL1 was mainly localized in the nucleus.Tissue expression profiles suggested that circ_MBNL1 was mainly expressed in duck spleen,adipose and lung tissues.The bioinformatics analysis results indicated that circ_MBNL1 and the predicted target genes were mainly enriched in adipocytokine signaling pathway,FoxO signaling and other pathways,which was hypothesized that circ_MBNL1 might be involved in regulating the differentiation process of duck adipocytes.This study provides a theoretical basis for an in-depth investigation of the regulatory role of circ_MBNL1 in the differentiation process of duck adipocytes.

关 键 词：鸭 肌肉盲样剪接调节因子1(MBNL1) 脂肪细胞 功能预测 

分 类 号：S834[农业科学—畜牧学]