杨梅品种特异性荧光SSR分子标记数据库构建  

Construction of Specific Fluorescent-labeled SSR Marker Database of Chinese Bayberry(Morella rubra)Varieties

作　　者：巨鹏举 朱奕凡 赵岚[1] 汪国云 周超超 颜丽菊 柴春燕[4] 焦云 陈金辉 郭秀珠[7] 高中山[1,6] JU Peng-Ju;ZHU Yi-Fan;ZHAO Lan;WANG Guo-Yun;ZHOU Chao-Chao;YAN Li-Ju;CHAI Chun-Yan;JIAO Yun;CHEN Jin-Hui;GUO Xiu-Zhu;GAO Zhong-Shan(Institute of Fruit Science,Zhejiang University,Hangzhou 310058,China;Agricultural Technology Extension Service Center of Yuyao City,Yuyao 315400,China;Linhai Specialty Technology Promotion Station,Linhai 317000,China;Cixi Forestry Technology Extension Centre,Cixi 315300,China;Forestry Research Institute,Ningbo Academy of Agricultural Science,Ningbo 315040,China;Yuyao Characteristic Fruit Research Institute,Yuyao 315400,China;Zhejiang Subtropical Crops Research Institute,Wenzhou 325005,China)

机构地区：[1]浙江大学果树科学研究所,杭州310058 [2]余姚市农业技术推广服务总站,余姚315400 [3]临海市特产技术推广总站,临海317000 [4]慈溪市林特技术推广中心,慈溪315300 [5]宁波市农业科学研究院林业研究所,宁波315040 [6]余姚市南方特色果树研究所,余姚315400 [7]浙江省亚热带作物研究所,温州325005

出　　处：《农业生物技术学报》2023年第10期2209-2220,共12页Journal of Agricultural Biotechnology

基　　金：宁波市科技重大专项(2021Z008);余姚杨梅地理标志保护工程项目(2020)。

摘　　要：我国杨梅(Morella rubra)种质资源丰富,地方品系较多。尽管基于杨梅基因组已开发了大量SSR分子标记,但目前尚未形成一套统一用于杨梅品种资源区分鉴定的分子标记,种质资源遗传特异性数据管理较为混乱。本研究基于已开发的杨梅SSR分子标记及连锁图谱定位,从每个连锁群中选择2个扩增片段长度稳定、重复性好的SSR分子标记,采用荧光标记引物,于DNA测序仪上进行片段分析,构建分子标记数据库,形成区分杨梅资源的统一标准;在此基础上分析杨梅各株系的遗传距离,绘制基于遗传距离的聚类树。结果显示,所选取的16个SSR分子标记在杨梅品系材料中的扩增片段大小存在差异,可用于109个杨梅品种与品系的区分,且不同品种与品系的地理来源与遗传距离的远近存在一定程度上的相关性;以地方主栽品种’荸荠种’、’东魁’、’丁岙’和’安海片早生’为扩增片段大小的标准参照,利用16个SSR分子标记的扩增片段构建109份杨梅资源的指纹图谱;进一步选择多态性信息含量(PIC)较高的5个SSR分子标记,可成功区分85%(94/109)的杨梅材料,显示较高的鉴定效率。本研究为杨梅种质资源管理提供更加高效、可靠的方法和标准。There are abundant germplasm resources of Chinese bayberry(Morella rubra)in China,and most of them are local accessions.Although a large number of SSR molecular markers have been developed based on the whole genome,a unified set of molecular markers for the identification of bayberry germplasm resources has not been established,which hampers the proper management of genetic identities of the germplasm.In this study,based on the developed SSR molecular markers and linkage map location of Chinese bayberry,2 SSR markers with stable amplicon length and good reproducibility were selected from each linkage group.The fluorescence-labeled primers were used and the amplified fragments were analyzed by DNA sequenator.Then the data was used to construct a molecular marker database and establish unified standard for the identification of Chinese bayberry resources.Afterwards,the genetic distances of every 2 accessions were calculated,and the neighbor-joining(NJ)clustering tree was drawn.The results showed that the amplified fragments of 16 SSR molecular markers were different and could distinguish 109 variety accessions.The geographical sources of the accessions were correlated with the genetic distance to a certain extent.A molecular database containing 109 accessions was established based on 16 SSR markers with'Biqi','Dongkui','Dingao'and'Anhaipianzaosheng'as reference marker of amplicon length.Further selection of 5 SSR molecular markers with high polymorphism information content(PIC)could successfully distinguish 85%(94/109)of Chinese bayberry materials,indicating high identification efficiency.This study provides a more efficient and reliable method and standard for the management of bayberry germplasm resources.

关 键 词：杨梅 指纹图谱 SSR 品种鉴定 聚类分析 

分 类 号：S324[农业科学—作物遗传育种]