复方木香保健酒对脾虚大鼠的影响  

Effect of Compound Aucklandia lappa Health Wine on Rats with Spleen Deficiency

作　　者：何枭 杜鹏 周小雪 张雪[2] 郑红 杨宪 HE Xiao;DU Peng;ZHOU Xiaoxue;ZHANG Xue;ZHENG Hong;YANG Xian(Engineering Research Center for Biotechnology of Active Substances(Ministry of Education),College of Life Sciences,Chongqing Normal University,Chongqing 40133l;College of Pharmacy,Chongqing Medical and Pharmaceutical College,Chongqing 401331,China)

机构地区：[1]重庆师范大学生命科学学院活性物质生物技术教育部工程研究中心,重庆401331 [2]重庆医药高等专科学校药学院,重庆401331

出　　处：《重庆师范大学学报（自然科学版）》2023年第4期143-153,共11页Journal of Chongqing Normal University:Natural Science

基　　金：国家自然科学基金面上项目(No.11572063);重庆市科学技术局基础研究与前沿探索项目(No.cstc2018jcyjAX0816);重庆市卫生健康委员会中医药科学技术项目(No.ZY201802053);重庆医药高等专科学校人才引进项目(No.ygz2018307);重庆市教育委员会科学技术研究项目(No.KJQN202100545);重庆市自然科学基金面上项目(No.cstc2021jcyj-msxmX0377)。

摘　　要：为探究复方木香保健酒对脾虚大鼠(Rattus norvegicus)的影响,选取SPF级SD大鼠,采用皮下注射利血平结合不规则喂养构建大鼠脾虚模型,并在造模成功后设置6个处理组,每组含10只大鼠,雌雄各半,其中:正常组(N组)保持正常喂养方式,模型组(T0组)保持脾虚状态,且均不予以药物治疗;莫沙必利组(TM组)予以每1 kg体质量0.01 g的枸橼酸莫沙必利,低、中、高剂量组(T1、T2和T3组)的给药量分别为每1 kg体质量给予10.0、5.0和2.5 mL的复方木香药液(其中药物含量相当于3.00 g·mL^(-1)的生药材)。连续给药14 d后,采集大鼠血样并进行有关血液指标的测定。结果显示:1) T0组大鼠精神萎靡、扎堆、拱背、皮毛枯槁、嗜睡等症状依然明显;T1、T2、T3和TM组大鼠的脾虚症状均有所减轻,与T0组相比体质量增长率和食物利用率有所提高。2)经过药物治疗,脾虚大鼠血液中的血小板计数得到提升,白细胞计数和总蛋白质量浓度与正常大鼠无明显差异;但血红蛋白质量浓度与红细胞计数未恢复。3) N组与T0组相比血清D-木糖质量浓度有统计学意义上的升高(p<0.05),T3组与N组的该指标差异无统计学意义,但与T0组的该指标差异有统计学意义(p<0.05)。N组与T0组相比血清免疫球蛋白G质量浓度有统计学意义上的升高(p<0.05),但4个药物治疗组与N组的该指标差异无统计学意义。N、T3组与T0组相比血清胃动素均有统计学意义上的升高(p<0.01),N、T3组雄性个体间的该指标差异无统计学意义但雌性个体间的该指标差异具有统计学意义(p<0.05);N组与其他5组相比血清胃泌素质量浓度均有统计学意义上的升高(p<0.05),TM、T3组与T0组相比该指标也有统计学意义上的升高(p<0.05)。N组与T0组的血清血管活性肠肽质量浓度差异具有统计学意义(p<0.01);在雄性个体间,TM、T3组与T0组相比血清血管活性肠肽质量浓度均有统计学意义上的升高(p<0.05),In order to explore the effect of compound Aucklandia lappa health wine on rats(Rattus norvegicus)with spleen deficiency,SPF SD rats were selected and the rat model of spleen deficiency was constructed by subcutaneous injection of reserpine combined with irregular feeding.SPF SD rats were selected,subcutaneously injected with reserpine combined with irregular feeding to replicate the spleen deficiency model.After successful modeling,6 treatment groups were set up,each containing 10 rats,with half male and half female,in which:the normal group(N group)maintained the normal feeding mode,and the model group(T0 group)maintained the state of splenic deficiency,and none of them was treated with medication;the mosapride group(TM group)was administered with 0.01 g of mosapride citrate per 1 kg of body mass.The dosages of low,medium,and high dose groups(T1,T2,and T3 groups)were 10.0,5.0,and 2.5 mL of compound A.lappa health wine per 1 kg body mass,respectively(the drug content was equivalent to the mass concentration of raw medicinal materials of 3.00 g·mL^(-1)).After 14 days of continuous administration,blood samples were collected from rats and the relevant blood indexes were measured.The results showed that:1)the rats in the T0 group had still obvious symptoms such as depression,clustering,arched back,withered fur and lethargy;the spleen deficiency symptoms of T1,T2,T3,and TM groups of rats were alleviated,and the growth rate of body mass and food utilization rate increased compared with those in the T0 group.2)After drug treatment,the platelet count in the blood of splenic asthenia rats was increased,and the white blood cell count and total protein mass concentration were not significantly different from those of normal rats;however,hemoglobin mass concentration and erythrocyte count were not restored.3)Serum D-xylose mass concentration in group N was significantly higher than that in group T0(p<0.05),and there was no significantly different between group T3 and group N,but there was significantly different between g

关 键 词：复方木香 脾虚模型 胃动素 胃泌素 血管活性肠肽 

分 类 号：TQ461[化学工程—制药化工] R285.1[医药卫生—中药学]