白斑狗鱼早期发育阶段的耐热性及耐热性状关联SNP筛选  

Heat resistance and screening of heat resistance-associated SNPs in the early development stage of Esox lucius

作　　者：海萨·艾也力汗[1] 贺疆滔 张钰[1] 李晓东[1] 沈玉帮[2] HAYSA·Ayelhan;HE Jiangtao;ZHANG Yu;LI Xiaodong;SHEN Yubang(Xinjiang Fishery Research Institute,Urumqi 830000,China;College of Fisheries and Life Science,Shanghai Ocean University,Shanghai 201306,China)

机构地区：[1]新疆维吾尔自治区水产科学研究所,新疆维吾尔自治区乌鲁木齐830000 [2]上海海洋大学水产与生命学院,上海201306

出　　处：《中国水产科学》2023年第6期677-684,共8页Journal of Fishery Sciences of China

基　　金：国家自然科学基金项目(32060826)。

摘　　要：为了解白斑狗鱼(Esox lucius)早期不同发育阶段的热耐受性,为其耐热性状的改良提供合适的分子标记,本研究首先采用耐热性(upper thermal tolerance,UTT)作为评定指标,对UTT进行方差分析,比较分析了白斑狗鱼不同发育阶段的热耐受性。使用GBS (genotyping-by-sequencing)技术获得两个极端组的耐热性状相关简化基因组测序数据集,进而对耐热性状进行全基因组关联分析(genome-wide association study,GWAS),发掘白斑狗鱼耐热性状关联的SNP标记。结果发现,白斑狗鱼早期不同发育阶段的耐热性有极显著的差异(P<0.01),幼鱼期的耐热性最高,其次为稚鱼期,仔鱼期耐热性最低。联合Farm CPU和Blink模型进行了GWAS分析,发现5个SNPs与白斑狗鱼耐热性状的关联性均达到了极显著水平(FDR P-value<0.01),分别位于clstn2、MAPK14、VAPA等基因的内含子和ANKS1B基因5’端非翻译区。这些基因直接或间接与刺激和应激反应相关。本研究初步筛选出与白斑狗鱼耐高温性状关联的候选SNPs和相关基因,为今后研究白斑狗鱼在应对高温胁迫时的生理机制和分子机制提供参考,为白斑狗鱼耐高温性状的改良提供了科学依据。To understand the heat tolerance of Esox lucius across different stages of early development and to elucidate appropriate molecular markers for the improvement of heat tolerance traits,this study used upper thermal tolerance(UTT)as an evaluation index,conducted variance analysis of UTT,compared and analyzed the thermal tolerance of Esox Lucius at different developmental stages,and used genotyping-by-sequencing(GBS)to obtain heat tolerance traits that were related to simplified genome sequencing data sets of two extreme groups.We then evaluated heat resistance using a genome wide association study to explore the SNP markers that are associated with heat tolerance traits in Esox lucius.The corresponding results showed that there were significant differences in heat tolerance at different developmental stages during the early development of Esox lucius(P<0.01).Heat tolerance was highest at the young stage,followed by the juvenile stage,with the lowest heat tolerance being observed at the larval stage.GWAS analysis,combined with FarmCPU and Blink models,showed that the correlations between the five SNPs and the heat tolerance traits of Esox lucius were highly significant(FDR P-value<0.01);these SNPs were located in the introns of clstn2,MAPK14,and VAPA genes,and the untranslated region at the 5ʹend of the ANKS1B gene.These genes are directly or indirectly associated with stimulus and stress responses.In this study,candidate SNPs and genes associated with high temperature tolerance traits were preliminarily screened in Esox Lucius.These results provide a reference for future studies regarding the physiological and molecular mechanisms of high temperature stress tolerance in Esox Lucius,and therefore provide a scientific basis for the improvement of high temperature tolerance in Esox lucius.

关 键 词：白斑狗鱼 早期发育阶段 耐热性 GWAS SNPS 

分 类 号：S917[农业科学—水产科学]