绞股蓝总苷对非酒精性脂肪性肝病小鼠肝脏脂质代谢物的干预作用  

Interventional Effect of Gypenosides on Liver Lipid Metabolism in Non-alcoholic Fatty Liver Disease Mice

作　　者：李德周[1] 奚瑛斐 辛鑫 李红山[1,3] LI Dezhou;XI Yingfei;XIN Xin;LI Hongshan(Department of Liver Disease,Ningbo No.2 Hospital,Ningbo Zhejiang 315010,China;Institute of Liver Disease,Shuguang Hospital,Shanghai University of Traditional Chinese Medicine,Shanghai 201203,China;Zhejiang Provincial Key Laboratory for Diagnosis,Treatment and Research of Digestive System Tumors,Ningbo Zhejiang 315010,China)

机构地区：[1]宁波市第二医院肝病科,浙江宁波315010 [2]上海中医药大学附属曙光医院肝病研究所,上海201203 [3]浙江省消化系统肿瘤诊治及研究重点实验室,浙江宁波315010

出　　处：《新中医》2023年第16期1-9,共9页New Chinese Medicine

基　　金：国家自然科学基金资助项目(81873109);宁波市重大科技专项项目(2022Z128)。

摘　　要：目的:基于脂质组学技术探讨绞股蓝总苷对非酒精性脂肪性肝病小鼠肝脏脂质代谢物的干预作用。方法:采用高脂饮食14周诱导小鼠(24只)非酒精性脂肪性肝病模型。在造模第11周起,随机分为模型组、绞股蓝总苷组、奥贝胆酸组,灌胃给药4周。8只小鼠作为正常组给予正常对照饲料。观察肝脏甘油三酯(TG)含量,肝组织病理变化[行苏木精-伊红染色法(HE)染色并计算非酒精性脂肪性肝病活动性评分(NAS)积分],血清丙氨酸氨基转移酶(ALT)、天冬氨酸氨基转移酶(AST)、总胆固醇(TC)、TG、低密度脂蛋白胆固醇(LDL-C)、高密度脂蛋白胆固醇(HDL-C)、空腹血糖、空腹胰岛素以及胰岛素抵抗指数的变化。应用液相色谱-质谱法(LC-MS)检测小鼠肝脏脂质代谢物。结果:模型组肝组织脂肪变性显著,肝组织TG、NAS积分、血清ALT、AST、TC、TG、LDL-C、空腹血糖、空腹胰岛素、胰岛素抵抗指数较正常组均上升(P<0.05);绞股蓝总苷组与奥贝胆酸组肝组织病理改变明显减轻,肝组织TG、NAS积分、血清AST、ALT、TC、空腹血糖、空腹胰岛素、胰岛素抵抗指数较模型组均降低(P<0.05);脂质组学结果显示,经绞股蓝总苷干预后,非酒精性脂肪性肝病小鼠FA、MGDG、PA、PC、PE、PS、LPE、LPC、SM等共17种肝脏脂质代谢物发生显著变化。结论:绞股蓝总苷可改善非酒精性脂肪性肝病脂质代谢紊乱。Objective:To explore the interventional effect of gypenosides on liver lipid metabolism in non-alcoholic fatty liver disease mice based on lipidomics technology.Method:A high-fat diet was used to induce a mouse model(n=24)of non-alcoholic fatty liver disease for 14 weeks.Starting from the eleventh week of modeling,they were randomly divided into model group,gypenosides group and obeticholic acid group,and administered by gavage for 4 weeks.Eight mice were given normal control feed as a normal group.Observe the content of triglycerides(TG)in the liver and the pathological changes in liver tissue[HE staining and calculate the non-alcoholic fatty liver disease Activity Score(NAS)],changes of serum alanine aminotransferase(ALT),aspartate aminotransferase(AST),total cholesterol(TC),TG,low-density lipoprotein cholesterol(LDL-C),high-density lipoprotein cholesterol(HDL-C),fasting blood glucose,fasting insulin and insulin resistance index.Application of liquid chromatography-mass spectrometry(LC-MS)was used to detect lipid metabolism in mouse liver.Results:The steatosis of liver tissue in the model group was significant.The TG and NAS scores of liver tissue,serum ALT,AST,TC,TG,LDL-C,fasting blood glucose,fasting insulin,and insulin resistance index were significantly higher than those in the normal group(P<0.05).The pathological changes of liver tissue in the gypenosides group and the obeticholic acid group were significantly reduced,and the TG and NAS scores of liver tissue,serum AST,ALT,TC,fasting blood glucose,fasting insulin,and insulin resistance index were significantly reduced compared to the model group(P<0.05).Lipidomics results showed that after intervention with gypenosides,there were significant changes in 17 liver lipid metabolism in non-alcoholic fatty liver disease mice,including FA,MGDG,PA,PC,PE,PS,LPE,LPC,and SM,etc.Conclusion:Gypenosides can improve lipid metabolic disorders in non-alcoholic fatty liver disease.

关 键 词：非酒精性脂肪性肝病 绞股蓝总苷 脂质代谢 脂质组学 

分 类 号：R528.5[医药卫生—内科学]