2021-2022年流感监测季北京市乙型Victoria系流感病毒血凝素基因特性和抗原性分析  

Phylogenetic and antigenic analysis of HA gene of influenza virus B(Victoria)in Bejing during 2021-2022surveillanceseason

作　　者：卢桂兰[1] 崔淑娟[1] 赵佳琛[1] 刘医萌[1] 石伟先[1] 冯兆民 潘阳[1] 张代涛[1] 杨鹏[1] 王全意[1] 彭晓旻[1] Lu Guilan;Cui Shujuan;Zhao Jiachen;Liu Yimeng;Shi Weixian;Feng Zhaomin;Pan Yang;Zhang Daitao;Yang Peng;Wang Quanyi;Peng Xiaomin(Institute for Infectious Disease and Endemic Disease Control,Beijing Center for Disease Prevention and Control,Beijing 100013,China)

机构地区：[1]北京市疾病预防控制中心传染病地方病控制所,北京100013

出　　处：《中华微生物学和免疫学杂志》2023年第8期619-626,共8页Chinese Journal of Microbiology and Immunology

摘　　要：目的:分析2021-2022年流感监测季北京市乙型Victoria系流感病毒(BV)的血凝素(hemagglutinin, HA)基因特性、抗原性及与流感疫苗组分株的匹配性。方法:采集2021-2022年流感监测季流感样病例(influenza like-illness, ILI)咽拭子样本经MDCK和鸡胚培养分离流感病毒,提取病毒核酸后测序。应用MEGA5.0进行病毒HA基因的核苷酸和氨基酸变异,采用maximum likelihood方法构建HA基因的遗传进化树,在线预测N-糖基化位点。SWISS-MODEL同源建模,建立BV毒株HA的蛋白质三维空间模拟图。通过血凝抑制(hemagglutination inhibition, HI)试验,进行毒株的抗原性分析。结果:共收集402株BV毒株,选取58株测序获得HA基因全长序列,与本年度的疫苗组分BV株(B/Washington/02/2019)HA基因相比较显示,有27个氨基酸位点发生变异,其中11个变异位点位于4个不同的抗原决定簇。遗传进化树分析显示:3个进化分支的毒株共同流行,其中54株(54/58,93.10%)HA基因位于Clade 1A.3a2分支,2株(2/58,3.45%)位于Clade 1A.3a1分支上,2株(2/58,3.45%)与2021-2022年度疫苗组分BV株在同一分支Clade 1A.3。其中2株相对于疫苗组分BV株增加一个197位糖基化位点,其余56株的糖基化位点未发现变化。抗原性分析结果显示:35株为疫苗组分BV株的类似株(35/58, 60.34%),23株为低反应株(23/58,39.66%)。结论:2021-2022流感监测季有3个进化分支的BV毒株在北京市人群中共同流行,其中Clade 1A.3a2毒株为优势株(93.10%),且与疫苗组分BV株进化距离有一定的偏离;占比近四成(39.66%)的毒株为疫苗低反应株。本研究结果提示,持续监测流感流行毒株的变异,及时为疫苗组分株筛选提供实验室依据,是应对流感大流行的基本技术保证。Objective To investigate the phylogenetic and antigenic characteristics of hemagglutinin(HA)gene of influenza B/Victoria lineage(BV)viruses in Beijing during the 2021-2022 influenza surveillance season,and to analyze whether the circulating BV viruses match the vaccine strain.Methods Pharyngeal swab specimens from influenza like-illness(ILI)cases in the 2021-2022 influenza surveillance season were collected from surveillance network labs in Beijing and cultured in MDCK cells and chicken embryo to isolate BV viruses.Nucleic acids of the viruses were extracted,and the HA gene was amplified and sequenced.The nucleotide and amino acid sequence identity of the HA gene was analyzed using MEGA5.0 software.A phylogenetic tree of HA gene was constructed using the maximum likelihood method.The N-glycosylation sites in HA were predicted online.Three-dimensional structure of HA was constructed using SWISS-MODEL homologous modeling.Hemagglutination inhibition(HI)test was performed to analyze the antigenicity of BV viruses.Results A total of 402 BV viruses were collected and 58 strains with full-length HA gene sequences were chosen for further analysis.Compared with the HA gene of this year's vaccine strain(B/Washington/02/2019),there were 27 amino acid mutations,11 of which were located in four different antigenic determinants.The phylogenetic analysis revealed that three subgroups of 1A.3,1A.3al,and 1A.3a2 co-circulated in Beijing with 54 strains(54/58,93.10%)clustered to the Clade 1A.3a2,two strains(2/58,3.45%)clustered to the Clade 1A.3al,and two strains(2/58,3.45%)in the same subgroup(Clade 1A.3)as the vaccine component BV strain in 2021-2022.Compared with the vaccine strain(B/Washington/02/2019),two BV strains had an additional N-glycosylation site at residue 197,while the other 56 strains showed no change in N-glycosylation sites.Antigenic analysis showed that 35 BV strains(35/58,60.34%)were antigenically similar to the vaccine strain and 23 strains(23/58,39.66%)were low-response strains.Conclusions Three subgroups of B

关 键 词：乙型流感病毒 HA基因 基因变异 遗传进化分析 抗原性分析 

分 类 号：R511.7[医药卫生—内科学]