用于MALDI-TOF MS鉴定的真菌蛋白质提取方法的研究进展  

Progress in protein extraction methods for fungal identification with MALDI-TOF MS

作　　者：王佳音 孙泽宇 许建成[1] Wang Jiayin;SunZeyu;Xu Jiancheng(Department of Laboratory Medicine,the First Hospital of Jilin University,Changchun 130021,China)

机构地区：[1]吉林大学第一医院检验科,长春130021

出　　处：《中华微生物学和免疫学杂志》2023年第8期647-652,共6页Chinese Journal of Microbiology and Immunology

摘　　要：随着微生物快速鉴定技术日趋兴起,MALDI-TOF MS已成为临床鉴定真菌的重要工具。蛋白质提取方法的适用性及标准化等问题阻碍MALDI-TOF MS技术在真菌领域的发展。本文分析真菌细胞壁复杂结构,介绍MALDI-TOF MS商业质谱系统推荐的蛋白质提取方法,整理MALDI-TOF MS鉴定各种属酵母样真菌和丝状真菌的蛋白质提取方法,如直接涂抹法、甲酸乙腈萃取法、磁珠研磨法等,总结目前MALDI-TOF MS鉴定真菌时蛋白质提取方法应用现状及积弊,以期为后来相关研究者提供理论参考。With the development of techniques for rapid microbial identification,MALDI-TOF MS has become an important tool for clinical identification of fungi.Problems such as the applicability and standardization of protein extraction methods have hindered the development of MALDI-TOF MS technology in the fungal field.This paper analyzed the complex structure of fungal cell walls,introduced the protein extraction methods recommended by MALDI-TOF MS commercial mass spectrometry systems,discussed the protein extraction methods for the identification of various genera of yeast-like fungi and filamentous fungi by MALDI-TOF MS,such as direct smear method,formic acid acetonitrile extraction method and magnetic bead grinding method,and summarized the current status and drawbacks of protein extraction methods in fungal identification by MALDI-TOF MS with a view to providing theoretical reference for subsequent research.

关 键 词：MALDI-TOF MS 蛋白质提取方法 真菌细胞壁 酵母样真菌 丝状真菌 

分 类 号：R446.5[医药卫生—诊断学]