Detection of neuronal defensive discharge information transmission and characteristics in periaqueductal gray double-subregions using PtNP/PEDOT:PSS modified microelectrode arrays  

作　　者：Botao Lu Penghui Fan Ming Li Yiding Wang Wei Liang Gucheng Yang Fan Mo Zhaojie Xu Jin Shan Yilin Song Juntao Liu Yirong Wu Xinxia Cai 

机构地区：[1]State Key Laboratory of Transducer Technology,Aerospace Information Research Institute,Chinese Academy of Sciences,Beijing 100190,China [2]University of Chinese Academy of Sciences,Beijing 100049,China

出　　处：《Microsystems & Nanoengineering》2023年第3期319-331,共13页微系统与纳米工程（英文）

基　　金：This work was sponsored by the National Natural Science Foundation of China(T2293731,L2224042,61988102,62121003,61960206012,62171434,61971400,61975206,and 61973292);the Frontier Interdisciplinary Project of the Chinese Academy of Sciences(XK2022XXC003);STI 2030-Major Projects 2021ZD0201600;the National Key R&D Program of China(2022YFC2402501);the Scientific Instrument Developing Project of the Chinese Academy of Sciences(GJJSTD20210004).

摘　　要：Threatened animals respond with appropriate defensive behaviors to survive.It has been accepted that midbrain periaqueductal gray(PAG)plays an essential role in the circuitry system and organizes defensive behavioral responses.However,the role and correlation of different PAG subregions in the expression of different defensive behaviors remain largely unexplored.Here,we designed and manufactured a microelectrode array(MEA)to simultaneously detect the activities of dPAG and vPAG neurons in freely behaving rats.To improve the detection performance of the MEAs,PtNP/PEDOT:PSS nanocomposites were modified onto the MEAs.Subsequently,the predator odor was used to induce the rat’s innate fear,and the changes and information transmission in neuronal activities were detected in the dPAG and vPAG.Our results showed that the dPAG and vPAG participated in innate fear,but the activation degree was distinct in different defense behaviors.During flight,neuronal responses were stronger and earlier in the dPAG than the vPAG,while vPAG neurons responded more strongly during freezing.By applying high-performance MEA,it was revealed that neural information spread from the activated dPAG to the weakly activated vPAG.Our research also revealed that dPAG and vPAG neurons exhibited different defensive discharge characteristics,and dPAG neurons participated in the regulation of defense responses with burst-firing patterns.The slow activation and continuous firing of vPAG neurons cooresponded with the regulation of long-term freezing responses.The results demonstrated the important role of PAG neuronal activities in controlling different aspects of defensive behaviors and provided novel insights for investigating defense from the electrophysiological perspective.

关 键 词：MICROELECTRODE FREEZING MODIFIED 

分 类 号：TB383[一般工业技术—材料科学与工程]