Spike-and nucleocapsid-based gold colloid assay toward the development of an adhesive bandage for rapid SARS-CoV-2 immune response detection and screening  

作　　者：Imen Boumar Muhammedin Deliorman Pavithra Sukumar Mohammad A.Qasaimeh 

机构地区：[1]Division of Engineering,New York University Abu Dhabi(NYUAD),Abu Dhabi,UAE [2]NYU Tandon School of Engineering,New York University,New York,USA

出　　处：《Microsystems & Nanoengineering》2023年第3期371-386,共16页微系统与纳米工程（英文）

基　　金：This work was financially supported by the NYUAD COVID-19 Facilitator Research Fund from NYU Abu Dhabi,UAE.The authors acknowledge the donors and nurses for their contributions to blood donation and collection.Additionally,the authors acknowledge the technical support from the Core Technology Platforms at NYU Abu Dhabi,especially the help of Mr.Vijay Dhanvi and Mr.Jinumon Govindan.The authors also thank Dr.Ayoub Glia for the discussions.

摘　　要：Immunoglobulin M(IgM)and immunoglobulin G(IgG)antibodies are important biomarkers used for the diagnosis and screening of severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)infections in both symptomatic and asymptomatic individuals.These antibodies are highly specific to the spike(S)and nucleocapsid(N)proteins of the SARS-CoV-2 virus.This paper outlines the development steps of a novel hybrid(vertical-lateral-vertical)flow assay in the form of a finger-stick point-of-care device,similar to an adhesive bandage,designed for the timely detection and screening of IgM and IgG immune responses to SARS-CoV-2 infections.The assay,comprising a vertically stacked plasma/serum separation membrane,conjugate pad,and detection(readout)zone,utilizes gold nanoparticles(AuNPs)conjugated with SARS-CoV-2 S and N proteins to effectively capture IgM and IgG antibodies from a pinprick(~15µL)of blood in just one step and provides results of no immune IgM−/IgG−,early immune IgM+/IgG−,active immune IgM+/IgG+or immune IgM−/IgG+in a short amount of time(minutes).The adhesive bandage-like construction is an example of the design of rapid,low-cost,disposable,and easy-to-use tests for large-scale detection and screening in households.Furthermore,the bandage can be easily adjusted and optimized to detect different viral infections as they arise by simply selecting appropriate antigens related to pandemics and outbreaks.

关 键 词：diagnosis utilize selecting 

分 类 号：TB383[一般工业技术—材料科学与工程]