中药注射剂类过敏反应的实验方法研究进展  

Advances in experimental methods of anaphylactoid reactions related to traditional Chinese medicine injections

作　　者：邓国艳 周鑫 刘红宇[1] 欧阳林旗[1,2] DENG Guoyan;ZHOU Xin;LIU Hongyu;OUYANG Linqi(Department of Pharmacy,The First Hospital of Hunan University of Chinese Medicine,Changsha 410007,China;School of Pharmacy,Hunan University of Chinese Medicine,Changsha 410208,China)

机构地区：[1]湖南中医药大学第一附属医院药学部,湖南长沙410007 [2]湖南中医药大学药学院,湖南长沙410208

出　　处：《药物评价研究》2023年第8期1787-1793,共7页Drug Evaluation Research

基　　金：国家自然科学基金资助项目(82174069);湖南省教育厅科学研究项目(21B0375);湖南省卫健委科研计划课题(C202313057956)。

摘　　要：中药注射剂(TCMIs)起效快、疗效确切、临床应用广泛,但其类过敏反应(ARs)等不良事件已成为中药安全性研究的热点和难点。近年来,为加快推进TCMIs安全性再评价,深入了解TCMIs相关ARs的症状特点、致敏成分、作用机制等,研究者采用体内实验(ARs症状观察法、伊文思蓝蓝染法、组织病理学检查法)、体外实验(甲基四氮唑与细胞计数试剂盒-8法、染色法、膜黏连蛋白V结合法等)方法对ARs进行了评估,可为建立可靠的评价方法提供依据,为临床TCMIs相关ARs的早期识别、风险防控提供新思路。Traditional Chinese medicine injections(TCMIs)have been widely used in clinical because of their rapid onset of action and precise efficacy,while their anaphylactoid reactions(ARs)and other adverse events have become a hot spot and a difficult area for safety research on traditional Chinese medicine.In recent years,in order to accelerate the safety re-evaluation of TCMIs and to gain a deeper understanding of the symptom characteristics,allergenic components and mechanisms of TCMIs-related ARs.The reseachers evaluated ARs using in vivo experiments(ARs symptom observation method,Evans blue staining method,histopathological examination method)and in vitro experiments(methyl tetrazolium and cell counting kit-8 method,staining method,membrane adhesion protein V binding method).It can provide a basis for establishing scientific and reliable evaluation methods,and provide new ideas for early identification,risk prevention and control of of clinical TCMIs-related ARs.

关 键 词：中药注射剂 类过敏反应 实验方法 安全性评价 组织病理学检查法 染色法 

分 类 号：R969.3[医药卫生—药理学] R285.5[医药卫生—药学]