不同处理措施对牛粪样中隐孢子虫卵囊杀灭效果观察  

Effect of different treatments on Cryptosporidium oocysts in cow dung samples

作　　者：江斌[1] 陈秀琴 吴胜会[1] 林琳[1] 张世忠[1] 蔡羲[1] Jiang Bin;Chen Xiuqin;Wu Shenghui;Lin Lin;Zhang Shizhong;Cai Xi(Institute of Animal Husbandry and Veterinary Medicine,Fujian Academy of Agricultural Sciences,Fuzhou350013)

机构地区：[1]福建省农业科学院畜牧兽医研究所,福州350013

出　　处：《福建畜牧兽医》2023年第5期1-2,共2页Fujian Journal of Animal Husbandry and Veterinary medicine

基　　金：福建省科技厅公益类项目(2021R1026002)资助。

摘　　要：取一例牛隐孢子虫阳性粪样分别采用1%甲醛溶液(A组)、1%二氯异氰脲酸钠溶液(B组)、2%烧碱溶液(C组)以及密封厌氧发酵(D组)处理,经5 d、10 d、15 d、20 d后分别取样采用蔗糖漂浮、涂片以及改良抗酸染色和镜检检查相应样本中隐孢子虫卵囊数量。结果A组4个时间节点卵囊数量分别为3.5枚、0.5枚、0枚、0枚;B组4个时间节点卵囊数量分别为1.75枚、0.25枚、0枚、0枚;C组4个时间节点卵囊数量分别为7.5枚、5.25枚、2.75枚、0.75枚;D组4个时间节点卵囊数量分别为9.75枚、6.50枚、4.75枚、3.00枚;而对照组4个时间节点卵囊数量分别为10.50枚、8.50枚、8.00枚、7.25枚。结果表明:4个试验组对牛隐孢子虫卵囊均有不同程度抑杀效果,其中B组效果相对较好。A bovine Cryptosporidium positive fecal sample was selected and treated with 1%formaldehyde solution,1%sodium dichloroisocyanurate solution,2%caustic soda solution and sealed anaerobic fermentation,respectively.After 5 d,10 d,15 d and 20 d,the samples were sampled respectively and tested for the number of Cryptosporidium oocysts in the corresponding samples by sucrose flotation,smear and modified acid-fast staining.Results showed that the number of oocysts at 4 time nodes in group A was 3.5,0.5,0 and 0,respectively.The number of oocysts at 4 time nodes in group B was 1.75,0.25,0 and 0,respectively.The number of oocysts at 4 time nodes in group C was 7.5,5.25,2.75 and 0.75,respectively.The number of oocysts at 4 time nodes in group D was 9.75,6.50,4.75 and 3.00,respectively.The number of oocysts at 4 time nodes in the control group was 10.50,8.50,8.00 and 7.25,respectively.The results showed that the four groups had different inhibitory effects on the oocysts of Cryptosporidium bovis,and the effect of group B was relatively better.

关 键 词：牛隐孢子虫 粪样 处理措施 卵囊 抑杀效果 

分 类 号：S855.9[农业科学—临床兽医学]