金叶含笑与2种含笑的杂交亲和性  

Compatibility between Michelia foveolata and two Michelia species

作　　者：张峥 邵凤侠 金晓玲 周宏香 张哲 李卫东[2] ZHANG Zheng;SHAO Fengxia;JIN Xiaoling;ZHOU Hongxiang;ZHANG Zhe;LI Weidong(College of Landscape Architecture,Central South University of Forestry and Technology,Changsha 410004,Hunan,China;Hunan Provincial Key Laboratory of Germplasm Innovation and Comprehensive Utilization of Garden Flowers,Hunan Horticulture Research Institute,Changsha 410125,Hunan,China)

机构地区：[1]中南林业科技大学风景园林学院,湖南长沙410004 [2]湖南省园艺研究所园林花卉种质创新与综合利用湖南省重点实验室,湖南长沙410125

出　　处：《浙江农林大学学报》2023年第5期961-969,共9页Journal of Zhejiang A&F University

基　　金：园林花卉种质创新与综合利用湖南省重点实验室开放课题(2019TP1033);湖南省重点研发计划项目(2021NK2008)。

摘　　要：【目的】探明金叶含笑Michelia foveolata与含笑M.figo和紫花含笑M.crassipes的杂交亲和性。【方法】以金叶含笑与含笑和紫花含笑作正反交授粉,于授粉2 h、4 h、6 h、8 h、10 h、12 h、1 d、2 d、4 d、6 d、8 d后,通过荧光显微镜观察花粉管的生长情况。同时展开金叶含笑与含笑和紫花含笑的正反交杂交试验,统计结实情况。【结果】①金叶含笑与含笑正反交的坐果率分别为75.0%和78.8%;②金叶含笑×紫花含笑坐果率(80.0%)显著高于紫花含笑×金叶含笑坐果率(6.7%);③含笑×金叶含笑花粉萌发较紫花含笑×金叶含笑晚2 h,花粉管在生长速度上无明显差异,均在8 d时进入胚珠;④以金叶含笑作母本的组合无明显杂交障碍,花粉管均在2~6 d内进入胚珠,种实发育良好;⑤含笑×金叶含笑存在花粉管在柱头表面杂乱生长、花粉管内部和先端有胼胝质堆积、花柱道细胞及间隙有大量胼胝质堆积等受精前障碍,虽不影响坐果,但对结籽有影响;⑥紫花含笑×金叶含笑存在受精前障碍和受精后障碍,受精前障碍包括花粉在柱头表面未萌发、花粉管在柱头表面盘旋生长未进入柱头、花粉管内部和先端堆积胼胝质而变得膨大,使花粉管生长受阻、花粉管在胚珠内弯曲缠绕成团,阻碍受精作用。但受精后障碍可能是影响落果的重要原因。【结论】金叶含笑与含笑正反交均表现亲和;金叶含笑×紫花含笑的亲和性程度高,紫花含笑×金叶含笑亲和性程度低。[Objective]This study is aimed to investigate the compatibility of Michelia foveolata with M.figo and M.crassipes.[Method]On the one hand,fluorescence microscope was used to observe the pollen tube growth after 2 h,4 h,6 h,8 h,10 h,12 h,1 d,2 d,4 d,6 d and 8 d.On the other hand,the reciprocal cross test was carried out of M.foveolata with M.figo and M.crassipes respectively to calculate the fruit and seed setting status.[Result](1)The fruit setting rates of the reciprocal cross of M.foveolata and M.figo were 75.0%and 78.8%respectively.(2)The fruit setting rate of M.foveolata×M.crassipes(80.0%)was significantly higher than that of M.crassipes×M.foveolata(6.7%).(3)The pollen germination of M.figo×M.foveolata was 2 h later than that of M.crasspies×M.foveolata and pollen tubes entered ovule on 8 d with no significant difference in growth rate.(4)There was no obvious hybridization barriers in the combination of M.foveolata as mother,and the pollen tube entered the ovule successively within 2 to 6 days with well developed fruit and seeds.(5)There were some pre-fertilization barriers,such as disarranged growth of pollen tube on stigma surface,callose accumulation in pollen tube,and callose accumulation in style channel cells and interstitial space and it was speculated that callose accumulation in style channel as the main cause of pre-fertilization barriers does not affect fruit setting,but has an effect on seed setting.(6)There were pre-fertilization and post-fertilization barriers in M.crassipes×M.foveolata.Pre-fertilization barriers include the failure of pollen to germinate on the stigma surface,the growth of pollen tubes in circles on the stigma surface,without entering the stigma,the expansion of pollen tubes due to the accumulation of callose inside and at the apex and the pollen tube becoming twisted and entangled within the ovule.However,post-fertilization barriers may be an important factor affecting fruit fall.[Conclusion]The reciprocal cross of M.foveolata and M.figo shows good compatibility with the c

关 键 词：金叶含笑 杂交亲和性 花粉管 坐果率 结籽率 

分 类 号：S685[农业科学—观赏园艺]