植物生长调节剂对杜仲叶片主要次级代谢产物的影响  

Effects of plant growth regulators on the main secondary metabolites in Eucommia ulmoides leaves

作　　者：周文玲 魏洪玲 李德文 唐中华[1,2,3] 刘英 解胜男[1,2] 田叙晨 储启明[1,2] ZHOU Wenling;WEI Hongling;LI Dewen;TANG Zhonghua;LIU Ying;XIE Shengnan;TIAN Xuchen;CHU Qiming(Key Laboratory of Forest Plant Ecology of Ministry of Education,Northeast Forestry University,Harbin 150040,Heilongjiang,China;College of Chemistry,Chemical Engineering and Resource Utilization,Northeast Forestry University,Harbin 150040,Heilongjiang,China;Heilongjiang Provincial Key Laboratory of Ecological Utilization of Forest-derived Active Substances,Northeast Forestry University,Harbin 150040,Heilongjiang,China)

机构地区：[1]东北林业大学森林植物生态学教育部重点实验室,黑龙江哈尔滨150040 [2]东北林业大学化学化工与资源利用学院,黑龙江哈尔滨150040 [3]东北林业大学黑龙江省林源活性物质生态利用重点实验室,黑龙江哈尔滨150040

出　　处：《浙江农林大学学报》2023年第5期999-1007,共9页Journal of Zhejiang A&F University

基　　金：黑龙江省自然科学基金资助项目(LH2021C014);东北林业大学中央高校基础研究项目(2572020BU02)。

摘　　要：【目的】探讨不同质量浓度植物生长调节剂对杜仲Eucommia ulmoides叶片次级代谢产物积累的影响,筛选促进次级代谢产物生成的最适植物生长调节剂配比条件,为杜仲次级代谢产物的调控提供参考。【方法】以2年生杜仲幼苗作为研究材料,施加不同质量浓度配比的吲哚乙酸(IAA)和6-苄氨基嘌呤(6-BA),测量杜仲叶片生长形态特征以及叶干、鲜质量,利用超高效液相色谱-质谱(UPLC-MS)测定9种次级代谢产物的质量分数,结合生物量进行主成分分析及因子分析,筛选高产量的次级代谢产物的最适生长调剂配比条件。【结果】与对照组相比,500 mg·L^(-1) IAA+500 mg·L^(-1)6-BA处理时,杜仲叶面积、叶周长、叶宽、叶干质量、叶鲜质量显著提高(P<0.05);相关性分析表明:IAA质量浓度与比叶面积、桃叶珊瑚苷、京尼平苷、京尼平苷酸质量分数呈显著正相关(P<0.05);6-BA质量浓度与绿原酸以及京尼平苷酸质量分数呈显著正相关(P<0.05);综合指标分析表明:单独施加500 mg·L^(-1)6-BA时,绿原酸、桃叶珊瑚苷、松脂醇二葡萄糖苷积累量最高,分别为0.036、0.046、0.031μg·g^(-1)。【结论】IAA质量浓度对桃叶珊瑚苷、车叶草苷酸积累作用显著;6-BA质量浓度对绿原酸、咖啡酸的积累作用显著。当复合施加100 mg·L^(-1) IAA+300 mg·L^(-1)6-BA时有利于植株的生长;施加100 mg·L^(-1) IAA+100 mg·L^(-1)6-BA时,不仅利于提高杜仲生物量,也促进次级代谢产物的积累。[Objective]The objective is to explore the effects of different concentrations of plant growth regulators on the accumulation of secondary metabolites in Eucommia ulmoides leaves and screen the optimal ratio conditions of plant growth regulator to promote the generation of secondary metabolites,so as to provide reference for the regulation of secondary metabolites in E.ulmoides.[Method]The 2-year-old E.ulmoides seedlings with different concentrations of 3-indole acetic acid(IAA)and 6-benzylaminopurine(6-BA)(15 groups) were used as materials, and growth morphological characteristics and dry and fresh weight of E. ulmoides leaves were measured. The contents of 9 secondary metabolites were determined by ultra high performance liquid chromatography-mass spectrometry (UPLC-MS). The optimal growth ratio conditions for high-yield secondary metabolites were selected by principal component analysis (PCA) and factor analysis combined with biomass. [Result] Compared with the control, the leaf area, leaf circumference, leaf width, dry and fresh weight significantly increased (P< 0.05) when the conditions of plant growth regulators was 500 mg·L^(-1) IAA + 500 mg·L^(-1) 6-BA. Correlation analysis showed that the IAA concentration was significantly positively correlated with leaf area, aucubin, geniposide and geniposidic acid (P<0.05). The concentration of 6- BA was significantly positively correlated with the content of chlorogenic acid and geniposidic acid (P<0.05). The comprehensive indicator analysis showed that when 500 mg·L^(-1) 6-BA was applied alone, the accumulation of chlorogenic acid, aucubin and pinoresinol diglucoside were the highest , which were 0.036, 0.046 and 0.031 μg·g−1 respectively. [Conclusion] The concentration of IAA has significant effects on the accumulation of aucubin and asperulosidic acid, while the concentration of 6-BA has significant effects on the accumulation of chlorogenic acid and caffeic acid (P<0.05). When 100 mg·L^(-1) IAA + 300 mg·L^(-1) 6-BA is applied in combination, it is bene

关 键 词：杜仲 植物生长调节剂 次级代谢产物 主成分分析 

分 类 号：S718.43[农业科学—林学]