Establishment and Application of A Human Primary Keratinocyte Inflammation Model for Cosmetic Raw Material Screening  

作　　者：Sun Fanghui Song Xiaojie Huo gang 

机构地区：[1]R&D Center,Osmun Biological Co.,Ltd.,China

出　　处：《China Detergent & Cosmetics》2023年第3期58-62,共5页日用化学品科学（英文版）

摘　　要：Using inducers to induce cells to produce inflammatory response is a common in vitro experimental method to study inflammation.However,there are relatively few inflammatory models developed for the cosmetic industry,and there are also great differences in the control of model induction,the selection of inflammatory indicators,and the concentration of inducers.Therefore,in this paper,by systematically studying the effects of Lipopolysaccharide(LPS)on the cell viability,the levels of IL-1α,IL-8 and ROS of human primary keratinocytes,a skin inflammation model based human primary keratinocyte was developed.The results showed that 0.01~100μg/mL LPS had no significant effect on the cell viability of human primary keratinocytes,while 100μg/mL LPS could simultaneously induce human primary keratinocytes to produce large amounts of IL-1αand IL-8,and 0.01μg/mL LPS could induce plentiful ROS.Therefore,a skin inflammation model for differential induction of different inflammatory indicators was established,and the sample OSM2021041301 was tested with this model,it was found that sample OSM2021041301 could significantly inhibit LPS-induced elevated IL-1αand IL-8 levels,the inhibitory effect on LPS-induced elevated ROS level was weak.The results indicated that OSM2021041301 has certain anti-inflammatory effect on inflammation caused by the increase of IL-1α,IL-8 and ROS induced by LPS and its analogues.

关 键 词：LPS human primary keratinocytes inflammation model IL-1Α IL-8 ROS 

分 类 号：TQ658[化学工程—精细化工]