Physicochemical Properties,Polysaccharide Composition Analysis and Efficacy Evaluation of Narcissus Tazetta Extract  

作　　者：Hsiao TzuChih Pan Fawu Wang Xili Gao Yuanyuan Lin Xiaofeng Chyau CharngCherng 

机构地区：[1]Juwenlee Cosmetics Technology Center,LUO LIH-FEN Group,China [2]Research Institute of Biotechnology,Hung Kuang University,China

出　　处：《China Detergent & Cosmetics》2023年第3期69-77,共9页日用化学品科学（英文版）

基　　金：Fujian Provincial Foreign Cooperation Project of Science and Technology Plan(Project No.:202111010)。

摘　　要：Numerous literature has reported that polysaccharides-based cosmetics present multifunctional effects in skin moisturizing for protecting the skin barrier.The aim of this study is to investigate the physicochemical properties,purities,composition and cellular functions of polysaccharides extracted from the bulb of Narcissus tazetta var.chinensis(NBP)and to evaluate its efficacy evaluation on skin moisture,elasticity,allergies and inflammation.The polysaccharides were obtained from the Narcissus tazetta bulb by using cold water extraction and ethanol precipitation methods.The residual impurities,polysaccharides molecular weight and composition were analyzed by UV spectrophotometry,gel chromatography,high performance liquid chromatography and infrared spectroscopy;skin hydration and elasticity assay were analyzed following efficacy evalution on human body;laboratory test was used to evaluate histamine and nitric oxide(NO)activity.The results indicate that a yield of 6%(w/w)of NBP,show that the NBP does not contain starch,protein,colchicine,lycorine,etc.,The average molecular weight of NBP is 559 kDa,and is composed of mannose and glucose,n(mannose):n(glucose)=4:1.0.5%NBP can increase skin hydration and elasticity assay on human body.The NBP is non-cytotoxic,in the basophils RBL-2H3 cell model tests,NBP concentrations at 100~250μg/mL can effectively inhibit the release of histamine,and the NBP concentrations 600~1000μg/mL can significantly inhibit LPS-induced NO production in RAW264.7 cells which are showed dose-responsive effects.These findings demonstrate that the NBP prepared in the study could offer potent skin protection,preserving hydration and elasticity,while also providing anti-allergy and anti-inflammatory properties.It can,therefore,be considered an excellent candidate for polysaccharide-based cosmetics.

关 键 词：HYDRATION ELASTICITY HISTAMINE nitric oxide 

分 类 号：TQ658[化学工程—精细化工]