miRNA-21的循环放大检测及成像  

Circulating Amplification Detection and Imaging of miRNA-21

作　　者：孙鑫鑫 李家家 丁彩凤[1] SUN Xinxin;LI Jiajia;DING Caifeng(College of Chemistry and Molecular Engineering,Qingdao University of Science and Technology,Qingdao 266042,China)

机构地区：[1]青岛科技大学化学与分子工程学院,山东青岛266042

出　　处：《青岛科技大学学报（自然科学版）》2023年第5期16-25,34,共11页Journal of Qingdao University of Science and Technology:Natural Science Edition

基　　金：国家自然科学基金项目(22074074);山东省自然科学基金项目(ZR2020MB065).

摘　　要：报道了一种miRNA循环放大检测系统,该系统涉及红色发射碳量子点(CDs)以及聚多巴胺纳米球(PDANSs)。通过纳米材料与DNA的自组装,在激发光源410、542 nm的照射下,产生650、585 nm的荧光信号,通过荧光信号的关-开,可以实现对靶标miRNA-21循环放大的双荧光检测与细胞成像,极大提高检测的灵敏度与准确性。This paper reports such a miRNA cycle amplification detection system,which involves red emitting carbon quantum dots(CDs)and polydopamine nanospheres(PDANSs).Through the self-assembly of nanomaterials and DNA,650 nm and 585 nm fluorescence signals are generated under the irradiation of 410 nm and 540 nm excitation light sources.Through the off-on of fluorescence signals,the double fluorescence detection and cell imaging of cyclic amplification of target miRNA-21 can be realized,which greatly improves the sensitivity and accuracy of detection.

关 键 词：碳量子点 聚多巴胺纳米球 MIRNA-21 循环放大检测 细胞成像 

分 类 号：O657.38[理学—分析化学]