生漆中C_(15)三烯漆酚的制备及对SKOV3细胞活力的影响  

Preparation of C_(15) triene urushiol in raw lacquer and its effect on SKOV3 cell viability

作　　者：朱志斌 李艳 吕虎强 张婷婷 李东旭 ZHU Zhi-bin;LI Yan;LV Hu-qiang;ZHANG Ting-ting;LI Dong-xu(Xi'an Lacquer Paint Research Institute of the All-China Federation of Supply and Marketing Cooperation,Xi'an 710061,China)

机构地区：[1]中华全国供销合作总社西安生漆涂料研究所,陕西西安710061

出　　处：《化学研究与应用》2023年第9期2249-2254,共6页Chemical Research and Application

基　　金：国家重点研发计划项目(2017YBFD0600705)资助;国际科技合作计划项目(S2021-YF-GHMS-0085)资助;陕西省自然科学基金项目(2021-NY-103)资助。

摘　　要：本研究以生漆为原料,乙醇提取、获得总漆酚乙醇提取物,采用高压制备液相色谱分离制备C_(15)三烯漆酚,使用HPLC、NMR进行纯度检测和结构表征,并采用MTT法研究C_(15)三烯漆酚对人卵巢癌细胞株SKOV3细胞增殖的抑制活性。结果制备获得一种三烯漆酚,经鉴定为3-(8’Z,11’E,13’Z-pentadecatrienyl) catechol,纯度大于97.5%,收率大于85.3%,MTT法体外抑制肿瘤增殖活性测试显示C_(15)三烯漆酚对人卵巢癌SKOV3细胞有较好的抑制活性,100μmol·L^(-1)的浓度下抑制率为49.97%,IC50值为71.38μmol·L^(-1)。In this study,raw lacquer was used as raw material to extract and obtain total urushiol ethanol extract.C_(15) triene urushiol was separated and prepared by high pressure preparation liquid chromatography.The purity and structure of C_(15) triene urushiol were determined by HPLC and NMR.MTT method was used to study the inhibitory activity of C_(15) triene urushiol on the proliferation of human ovarian cancer cell line SKOV3.Results A kind of triene urushiol was prepared,which was identified as 3-(8'Z.11'E,13'Zpentadecyclinyl) cathol.The purity was more than 97.5%,and the yield was more than 85.3%.MTT assay showed that C15 triene urushiol had good inhibitory activity on human ovarian cancer cell SKOV3 in vitro,the inhibitory rate was 49.97%,and the IC50 value was 71.38 μmol·L^(-1)

关 键 词：生漆 制备液相色谱 C_(15)三烯漆酚 分离纯化 SKOV3细胞活力 

分 类 号：O629.9[理学—有机化学]