LC-MS/MS法测定肝脏中甲卡西酮和卡西酮  

Determination of methcathinone and cathinone in liver by LC-MS/MS

作　　者：易荣楠 赵明明[1] 凌江[2] 陈志伟 刘冬娴[1] YI Rong-nan;ZHAO Ming-ming;LING Jiang;CHEN Zhi-wei;LIU Dong-xian(Criminal Technology Department,Hunan Police Academy,Changsha 410138,China;School of Basic Medical Science,Central South University,Changsha 410013,China)

机构地区：[1]湖南警察学院刑事科学技术系,湖南长沙410138 [2]中南大学基础医学院,湖南长沙410013

出　　处：《化学研究与应用》2023年第9期2268-2272,共5页Chemical Research and Application

基　　金：湖南省自然科学基金项目(2018JJ2109)资助;湖南省教育厅科学研究重点项目(21A0605)资助;湖南警察学院社会安全风险与治安治理科研创新团队资助。

摘　　要：为测定肝脏组织中甲卡西酮及其代谢物卡西酮,研究开发了液相色谱-串联质谱(LC-MS/MS)方法。肝脏组织剪碎、研磨成匀浆,添加含内标双苯戊二氨酯(SKF_(525A))的乙腈溶液,经涡旋、振荡、离心后,0.22μm微孔有机滤膜过滤,LC-MS/MS检测。采用ZORBAX SB-C_(18)色谱柱(50 mm×4.6 mm,1.8μm),柱温为23℃,乙腈和0.2%甲酸水溶液为流动相,流速为2.0 mL·min-1进行梯度洗脱。电喷雾离子源(ESI),正离子多反应监测模式(MRM)检测。结果表明该方法对甲卡西酮的检测在0.5~3000 ng·g^(-1)范围内呈现良好的线性关系,检出限为0.2 ng·g^(-1),定量限为0.5 ng·g^(-1);对卡西酮的检测在1.0~3000 ng·g^(-1)范围内呈现良好线性关系,检出限为0.5 ng·g^(-1),定量限为1.0 ng·g^(-1);甲卡西酮和卡西酮的回收率分别为91.51%~96.35%和90.55%~94.08%,精密度均小于15%。该方法操作简便、灵敏度较高,适用于肝脏组织中甲卡西酮及其代谢物卡西酮的浓度测定及代谢研究。A liquid chromatography-tandem mass spectrometry(LC-MS/MS)method was developed for the determination of methcathinone and its metabolite cathinone in liver.The liver tissue was ground into homogenate,and acetonitrile solution containing proadifen hydrochloride(SKF_(525A))was added.The sample was vortexed and centrifuged.The supernatant was separated and filtrated using 0.22μm Millipore filter before LC-MS/MS analysis.ZORBAX SB-C_(18)column(1.8μm,50 mm×4.6 mm)was used with column temperature of 23℃,mobile phase of acetonitrile and 0.2%formic acid in water,gradient elution,flow rate of 2.0 mL·min^(-1).Data was acquired using multiple reaction monitoring(MRM)mode with electrospray ion source(ESI).The calibration curves of methcathionone in liver tissue displayed good linear in the range of 0.5~3000 ng·g^(-1)with detection limit of 0.2 ng·g^(-1).The linear relationship of cathinone was in the range of 1.0~3000 ng·g^(-1)with detection limit of 0.5 ng·g^(-1).Their recoveries ranged from91.51%to 96.35%and 90.55%to 94.08%,respectively.The RSD was less than 15%.A simple and sensitive method was established to determine methcathinone and its metabolite cathinone in liver tissue.

关 键 词：液相色谱-串联质谱法 肝脏 甲卡西酮 卡西酮 

分 类 号：O657.7[理学—分析化学]