氢气和氧气共存下三氯乙烯高效降解菌Pseudomonas putida HO1的分离鉴定与降解特性  

Isolation,Identification and Degradation Characteristics of an Efficient Trichloroethene-degrading Bacterium Pseudomonas putida HO1 in the Presence of Hydrogen and Oxygen

作　　者：薛晨 李翠 刘明慧 余锦涛 欧阳葳葳 刘慧 XUE Chen;LI Cui;LIU Minghui;YU Jintao;OUYANG Weiwei;LIU Hui(School of Environmental Studies,China University of Geosciences(Wuhan),Wuhan 430078,China)

机构地区：[1]中国地质大学(武汉)环境学院,湖北武汉430078

出　　处：《安全与环境工程》2023年第5期258-265,共8页Safety and Environmental Engineering

基　　金：国家重点研发计划项目(2018YFC1802504);国家自然科学基金面上项目(42277051)。

摘　　要：三氯乙烯(TCE)是一种被广泛使用的有机物,不仅在环境中更稳定、更难降解,而且对人体健康具有潜在的风险。前期研究发现通过电化学体系向地下水环境中同时通入氢气(H_(2))和氧气(O_(2))能促进TCE的降解。通过持续通H_(2)、O_(2)及二氧化碳(CO_(2))气体的条件下,从氯代烃污染沉积物中富集、分离得到一株TCE降解菌(编号为HO1),通过对该降解菌HO1进行鉴定,研究其在H_(2)/O_(2)共存条件下对TCE的降解特性,并考察其对TCE污染沉积物的强化修复效果。结果表明:降解菌HO1细胞呈杆状,菌团呈大小均匀的浅粉色球状,鉴定为假单胞菌Pseudomonas putida,命名为Peudomonas putidaHO1;该菌株能利用所试的15种碳源生长;该菌株在纯O_(2)和H_(2)/O_(2)共存条件下能高效降解TCE,TCE的降解过程符合一级动力学模型,TCE降解的一级反应速率常数分别为(0.063 9±0.005 6) d^(-1)和(0.047 5±0.004 9) d^(-1);该菌株能同时利用体系中的H_(2)、O_(2)及CO_(2)合成自身有机物质,为细胞生长提供能量;在H_(2)/O_(2)共存的TCE污染沉积物体系中,加入该菌株能显著促进TCE的降解;在寡有机物的地下环境中,利用该菌株,通过通入H_(2)/O_(2)有望强化TCE污染沉积物的生物修复。该菌株可为实际场地微生物修复TCE提供可能的菌种资源。Trichloroethylene(TCE),a widely used organic matter,is not only more stable and more difficult to degrade in the environment but also a potential health risk to human health.Previous research has found that the introduction of hydrogen(H_(2))and oxygen(O_(2))into the ground water environment canpromote TCE degradation.A TCE-degrading bacterial strain is enriched and isolated from the chlorinated hydrocar-bons-contaminated sediment under continuous ventilation of H_(2),O_(2),and carbon dioxide(CO_(2)),numbered HO1.Through the identification of the degrading bacterium HOl,the degradation characteristics under the condition of H_(2)/O_(2) coexistence are studied,and its enhanced remediation effect on TCE-contaminated sed-iments is investigated.The strain HOl is rod-shaped,and the colonies are light pink spherical with uniform size.It is identified as Pseudomonas putida.The strain can use 15 kinds of carbon sources tested.It effi-ciently degrades TCE under pure O_(2) and H_(2)/O_(2) conditions.The degradation process fit the first-order ki-netic model,and the first-order reaction constants are(0.0639±0.0056)d^(-1) and(0.0475±0.0049)d^(-1),respectively,under two conditions.The strain can simultaneously use H_(2),O_(2),and CO_(2) in the system to synthesize organic matter for cell growth.The supply of this bacterial suspension to the TCE-contaminated sediment system can significantly promote TCE degradation under the coexistence of H_(2) and O_(2).There-fore,in the underground environment with little organic matter,supplying the bacterial strain and H_(2)/O_(2)is expected to enhance the bioremediation of TCE contamination.The strain provides a possible microbial re-source for microbial remediation of TCE in actual sites.

关 键 词：三氯乙烯(TCE) 污染沉积物 降解菌HO1 氢气 氧气 分离鉴定 降解 

分 类 号：X783[环境科学与工程—环境工程]