咖啡酸苯乙酯对大鼠早期腹主动脉瘤形成的抑制作用  

Inhibitory effect of caffeic acid phenethyl ester on early abdominalaortic aneurysm in rats

作　　者：占钻[1] 刘勇[1] 刘坚[1] 黄亮[1] 曹春水[1] ZHAN Zuan;LIU Yong;LIU Jian;HUANG Liang;CAO Chunshui(Department of Emergency,The First Affiliated Hospital of Nanchang University,Nanchang,Jiangxi 330006,China)

机构地区：[1]南昌大学第一附属医院急诊科,南昌330006

出　　处：《重庆医学》2023年第18期2741-2744,2750,共5页Chongqing medicine

基　　金：江西省教育厅科技项目(GJJ190042)。

摘　　要：目的 探讨咖啡酸苯乙酯(CAPE)对大鼠早期腹主动脉瘤(AAA)形成的抑制作用。方法 健康雄性SD大鼠分为模型组、CAPE干预组和假手术组,每组6只。采用弹性蛋白酶法建立AAA模型,CAPE干预组腹腔注射CAPE 10μmol·kg~(-1)·d~(-1),每日1次,持续14 d,模型组和假手术组在相同时间内注射等剂量生理盐水。观察AAA的形成、大小、血管壁组织病理改变;免疫组织化学法检测环氧合酶-2(COX-2)、前列腺素E2(PGE2)、基质金属蛋白酶(MMP)-2及MMP-9水平。结果 14 d后模型组、CAPE干预组大鼠AAA直径较假手术组腹主动脉直径增大[(3.32±1.23)mm vs.(2.42±0.14)mm vs.(1.47±0.08)mm,P<0.05],CAPE干预组较模型组减小(P<0.05)。HE染色显示,CAPE干预后瘤样扩张不明显,组织结构较模型组完整,有炎症浸润,但较模型组明显减少。VG染色显示,与模型组比较,CAPE干预组AAA血管壁肌纤维增加,胶原纤维断裂及降解情况好转。与假手术组比较,模型组AAA血管壁组织COX-2、PGE2、MMP-2及MMP-9水平均明显升高(P<0.05),CAPE干预后上述指标水平较模型组均明显降低(P<0.05)。结论 CAPE对大鼠早期AAA形成具有抑制作用,其可能与其抑制炎性反应,以及COX-2、PGE2和MMPs等水平相关。Objective To investigate the inhibitory effect of caffeic acid phenethyl ester(CAPE)on early abdominal aortic aneurysm(AAA)and its possible mechanism in rats.Methods A total of 18 healthy male SD rats were divided into the model group,the CAPE intervention group and the sham operation group,with six rats in each group.The AAA model was established by elastin enzyme method.The CAPE intervention group was intraperitoneally injected with CAPE 10μmol·kg-1·d-1 for 14 days.The model group and the sham operation group were injected with the same dose of normal saline at the same time.The formation,size and pathological changes of AAA were observed;The levels of cyclooxygenase-2(COX-2),prostaglandin E2(PGE2),matrix metalloproteinase(MMP)-2 and MMP-9 were detected by immunohistochemistry.Results The diameter of AAA after 14 days in the model group and the CAPE intervention group was significantly higher than that in the sham operation group[(3.32±1.23)mm vs.(2.42±0.14)mm vs.(1.47±0.08)mm,P<0.05],but the diameter of CAPE intervention group was significantly lower than that in the model group(P<0.05).HE staining showed that the tumor-like expansion was not obvious after CAPE intervention,and the tissue structure was more complete than that of the model group,with inflammatory infiltration,but it was significantly less than that of the model group.VG staining showed that compared with th e model group,the muscle fibers of the AAA vascular wall in the CAPE intervention groupwere significantly increased,and the collagen fibers were disordered and degraded better.Compared with the sham operation group,the levels of COX-2,PGE2,MMP-2 and MMP-9 in AAA vascular wall tissue in the model group were significantly increased(P<0.05).Conclusion CAPE can inhibit the early formation of AAA in rats,which may be related to its inhibition of inflammatory response and the levels of COX-2,PGE2 and MMPs.

关 键 词：咖啡酸苯乙酯 腹主动脉瘤 炎性反应 

分 类 号：R459.9[医药卫生—治疗学]