Plackett-Burman实验设计联用Box-Behnken响应面法优化柚皮素白蛋白纳米粒的处方工艺  

Optimization of Formulation and Process of Naringenin Albumin Nanoparticles Based on Plackett-Burman Coupled with Box-Behnken Response Surface Methodology

作　　者：赵林 隋硕 王建筑 ZHAO Lin;SUI Shuo;WANG Jian-zhu(School of Pharmaceutical Science,Shandong First Medical University&Shandong Academy of Medical Science,Taian 271000,China)

机构地区：[1]山东第一医科大学(山东省医学科学院),山东泰安271000

出　　处：《海峡药学》2023年第8期16-21,共6页Strait Pharmaceutical Journal

基　　金：山东省大学生创新创业训练计划项目(S201910439090)。

摘　　要：目的 优化NabTM技术制备柚皮素的牛血清白蛋白纳米粒的处方工艺。方法 以包封率为指标,采用Plackett-Burman实验设计结合Box-Behnken响应面法优化柚皮素白蛋白纳米粒的处方工艺,并考察纳米粒的体外释放规律。结果 最终确定柚皮素白蛋白纳米粒处方工艺为:白蛋白浓度为30 mg·mL-1,水相pH值为6,有机相与水相比例为1∶5,制备的纳米粒包封率为88.80%,粒径为171.9 nm, PDI为0.340,体外释放具有明显缓释作用。结论 Plackett-Burman实验设计联用Box-Behnken响应面法可用于优化柚皮素白蛋白纳米粒工艺处方。OBJECTIVE To optimize the formulation of bovine serum albumin nanoparticles(BSA)for naringenin preparation by Nab TM technology.METHODS The formulation and technology of naringenin albumin nanoparticles were optimized by Plackett Burman experimental design combined with Box Behnken response surface methodology,and the in vitro release of naringenin albumin nanoparticles was investigated.RESULTS The formulation of naringenin albumin nanoparticles was:the concentration of albumin was 30 mg·mL-1,the pH of aqueous phase was 6,and the ratio of organic phase to aqueous phase was 1∶5.The entrapment efficiency of the prepared nanoparticles was 88.80%,the particle size was 171.9 nm,and the PDI was 0.340.The in vitro release of the nanoparticles showed significant sustained release effect.CONCLUSION Plackett Burman experimental design combined with Box Behnken response surface methodology can be used to optimize the formulation of naringenin albumin nanoparticles.

关 键 词：PLACKETT-BURMAN设计 Box-Behnken响应面法 柚皮素 白蛋白纳米粒 

分 类 号：R94[医药卫生—药剂学]