白细胞过滤器对红细悬液中血小板的去除效率  

Platelet Removal Efficiency by Leukocyte Filter in Red Blood Cell Units at Different Storage Periods

作　　者：刘浩强 彭琳 林宇霆 冯宝莹[1] 巴桑 嘎玛格列 陆英[1,2] LIU Hao-qiang;PENG Lin;LIN Yu-ting;FENG Bao-ying;BA Sang;GAMA Ge-lie;LU Ying(Department of Blood Transfusion,The Third Affiliated Hospital,Sun Yat-sen University,Guangzhou 510630,China;Clinical Laboratory,Nyingchi People's Hospital,Nyingchi 860000,China)

机构地区：[1]中山大学附属第三医院输血科,广东广州510630 [2]西藏自治区林芝市人民医院检验科,西藏林芝860000

出　　处：《中山大学学报（医学科学版）》2023年第5期835-839,共5页Journal of Sun Yat-Sen University:Medical Sciences

基　　金：中山大学附属第三医院国家自然科学基金培育专项基金(2021GZRPYMS05);广州市科技计划项目(202206010195)。

摘　　要：【目的】观察不同储存时期红细胞悬液中血小板数量的变化,探讨白细胞过滤器对红细胞悬液中的血小板去除效率。【方法】将58份红细胞悬液根据储存时间不同分为4组:1周组(16份),2周组(16份),3周组(14份),4周组(12份),将四组红细胞悬液通过白细胞过滤器过滤,获取过滤前后的样本,用全自动血细胞计数仪检测血小板计数,计算出血小板的去除率。过滤前红细胞悬液标本制成血细胞涂片,用吉姆萨染色液染色,显微镜下观察血小板的形态。【结果】四组红细胞悬液中过滤前血小板的计数分别为(286.5±62.34)×10^(9)/L、(238.0±57.37)×10^(9)/L、(193.6±56.21)×10^(9)/L和(167.8±24.76)×10^(9)/L,储存3周(P<0.01)和4周(P<0.0001)组血液中的血小板计数明显低于储存1周组。不同储存时期红细胞悬液血细胞涂片中,均可以观察到成群分布和散在分布的形态正常的血小板。4组红细胞悬液的血小板去除率分别为(80.13±9.06)%,(76.41±10.13)%,(77.78±9.30)%和(70.63±9.39)%,各组之间血小板去除率比较差异无统计学意义(P>0.05)。【结论】红细胞悬液中的血小板计数随着储存时间的延长而逐渐降低,而储存后期(3周和4周)仍保存有大部分的血小板,白细胞过滤器能够去除其中的绝大多数血小板,而且各组间去除效率相似。【Objective】The aim of this study is to investigate change of platelet count in red blood cell(RBC)units at different storage periods and explore the efficiency of platelet removal by leukocyte filter.【Methods】A total of 58 RBC units were divided into four groups according to different storage periods:1 week Group(16),2 weeks Group(16),3 weeks Group(14)and 4 weeks Group(12).RBC units in the four groups were filtered through leukocyte filter.The RBC samples before and after filtration were obtained.The platelet count was detected by automatic blood cell counter and the ef⁃ficiency of platelet removal was calculated.RBC samples before filtration were made into blood cell smears.The blood cell smears were dyed with Wright-Giemsa stain,and the morphology of platelets was observed through a microscope.【Re⁃sults】The platelet count in RBC units stored for 1,2,3 and 4 weeks was(286.5±62.34)×10^(9)/L,(238.0±57.37)×10^(9)/L,(193.6±56.21)×10^(9)/L and(167.8±24.76)×10^(9)/L,respectively.Platelet count in blood stored for 3 weeks(P<0.01)and 4 weeks(P<0.0001)were significantly lower than those stored for 1 weeks.When observed in the blood smears of RBC units at different storage periods,platelets with normal morphology were distributed in clump and scattered style.The plate⁃let removal rates of the four groups were(80.13±9.06)%,(76.41±10.13)%,(77.78±9.30)%and(70.63±9.39)%,re⁃spectively,with no significant difference(P>0.05).【Conclusions】Platelet count in RBC units decreases gradually as the storage period increases,but most platelets still remain in RBC units of late storage periods(3 weeks and 4 weeks).The leukocyte filter is able to remove most of the platelets,and the removal efficiency is similar among the groups.

关 键 词：红细胞悬液 白细胞过滤器 储存时间 血小板去除 效率 

分 类 号：R446[医药卫生—诊断学]