一例树鼩毛发上皮瘤的诊断及细胞生物学特性观察  

Diagnosis of Trichoepithelioma in a Tree Shrew and Observation of Cell Biological Characteristics

作　　者：翟珊珊 梁亮[1] 曹颖颖 李竹欣 王青 陶俊宇 运晨霞[1,3] 冷静 唐海波[1,2] ZHAI Shanshan;LIANG Liang;CAO Yingying;LI Zhuxin;WANG Qing;TAO Junyu;YUN Chenxia;LENG Jing;TANG Haibo(Guangxi University of Chinese Medicine,Nanning 530200,China;Guangxi Key Laboratory of Translational Medicine for Treating High-Incidence Infectious Diseases with Integrative Medicine,Nanning 530200,China;Key Laboratory of Characteristic Experimental Animal Models of Guangxi,Nanning 530200,China)

机构地区：[1]广西中医药大学,南宁530200 [2]广西高发传染病中西医结合转化医学重点实验室,南宁530200 [3]广西特色实验动物模型重点实验室,南宁530200

出　　处：《实验动物与比较医学》2023年第4期440-445,共6页Laboratory Animal and Comparative Medicine

基　　金：广西自然科学基金项目“基于树鼩模型探索TLR8在狂犬病发病过程中的作用机理”(2020GXNSFAA297188);广西高等学校千名中青年骨干教师培育计划“广西中医药大学-唐海波”(第三期)[桂教师范(2019)81号];广西中医药大学高层次人才培育创新团队“冷静团队”(2022B006);2023年广西中医药多学科交叉创新团队项目“中医药生物新材料关键技术研究与智能装备开发学科交叉创新团队”(GZKJ2303)。

摘　　要：目的观察实验动物树鼩的原发性肿瘤,为研究毛发上皮瘤的发生机制及防治措施提供基础。方法将常规自然饲养过程中发现胸腹部长有肿块的1例树鼩麻醉后,手术摘除肿块,肿块组织经石蜡切片后行HE染色及免疫组织化学染色,并对肿瘤细胞进行分离和传代培养。分离获得的肿瘤细胞接种至健康树鼩及裸小鼠皮下。每天观察1次肿瘤细胞的体内成瘤情况,裸小鼠连续观察2个月,树鼩观察6个月以上。结果HE染色显示发病树鼩腹部肿块组织中真皮内基底样细胞整体排列似串花瓣样,形成巢状及条索状且边缘界限清晰的肿瘤;进一步放大倍数后观察发现肿瘤细胞呈栅栏状、基底状排列,核染色深,细胞质少。免疫组织化学染色显示,肿瘤细胞中CK蛋白强阳性表达,ki-67蛋白呈低比例阳性表达;肿瘤细胞间质中Vimentin强阳性表达,Bcl2和CD10呈低比例阳性表达。分离获得的肿瘤细胞在含10%胎牛血清的DMEM培养液中生长良好,可传代培养。健康树鼩及裸小鼠皮下接种肿瘤细胞后,均未见肿瘤形成。结论结合肿瘤发生部位、大体形态结构、病理切片HE染色及免疫组织化学结果,综合诊断该例树鼩胸腹部肿块为毛发上皮细胞瘤。Objective To observe the primary tumor of tree shrews and to provide a basis for studying the pathogenesis and prevention of trichoepithelioma.Methods A tumor was discovered in the chest and abdomen of a tree shrew during natural cultivation.The tree shrew was anesthetized,and the tumor was surgically removed.Hematoxylin and eosin(HE)staining and immunohistochemical staining were performed on the tumor tissue after paraffin section,and the tumor cells were isolated and cultured by passage.The isolated tumor cells were subcutaneously injected into healthy tree shrews and nude mice.The tumorigenesis of tumor cells in vivo was observed once a day,with nude mice continuously observed for 2 months and tree shrews observed for more than 6 months.Results HE staining showed that the basal cells in the dermis were arranged as a whole,like a string of petals,forming nests and stripe-like structures with clear boundaries.The observation results after magnification revealed that the tumor cells were arranged in a pallisade-like and basal pattern,with deep nuclear staining and minimal cytoplasmic.Immunohistochemical staining showed the high expression of CK protein and low proportion expression of ki-67 protein in tumor cells,as well as the high expression of vimentin and low expressions of Bcl2 and CD10 in tumor cell mesenchyme.The isolated tumor cells grew well in DMEM medium containing 10%fetal bovine serum and could be cultured by passage,but no tumor formation was observed in healthy tree shrews and nude mice inoculated with tumor cells.Conclusion Combined with the location of the tumor,overall morphology,HE staining,and immunohistochemical results,the thoracoabdominal mass of the tree shrew was diagnosed as a trichoepithelioma.

关 键 词：树鼩 毛发上皮瘤 诊断 肿瘤细胞移植 裸小鼠 

分 类 号：R-332[医药卫生] Q95-33[生物学—动物学]