海洋放线菌Actinoplanes sp.M4I6化学成分研究  

Study on the chemical constituents from the marine-derived actinomycete Actinoplanes sp.M4I6

作　　者：王子鹏 李芬发 谢晴宜[2] 马青云[2] 杨理 戴好富[2] 罗都强 赵友兴[2] WANG Zipeng;LI Fenfa;XIE Qingyi;MA Qingyun;YANG Li;DAI Haofu;LUO Duqiang;ZHAO Youxing(College of Life Science,Key Laboratory of Microbial Diversity Research and Application of Hebei Province,Hebei University,Baoding 071002,China;Haikou Key Laboratory for Research and Utilization of Tropical Natural Products,Institute of Tropical Bioscience and Biotechnology,Chinese Academy of Tropical Agricultural Sciences,Hainan Key Laboratory for Protection and Utilization of Tropical Bioresources,Hainan Academy of Tropical Agricultural Resource,Haikou 571101,China)

机构地区：[1]河北大学生命科学学院,河北省微生物多样性研究与应用重点实验室,河北保定071002 [2]中国热带农业科学院热带生物技术研究所海口市热带天然产物研究与利用重点实验室,海南热带农业资源研究院海南省热带农业生物资源保护与利用重点实验室,海南海口571101

出　　处：《热带海洋学报》2023年第5期38-44,共7页Journal of Tropical Oceanography

基　　金：海南省自然科学基金项目(821RC643);财政部和农业农村部国家现代农业产业技术体系专项(CARS-21);农业农村部财政专项(NFZX2021)。

摘　　要：对海洋放线菌Actinoplanes sp.M4I6的次生代谢产物进行分离鉴定,并评价了其生物活性。采用正相硅胶柱色谱、反相C18柱色谱、Sephadex LH-20凝胶柱色谱和高效液相色谱等技术进行分离纯化,运用核磁共振波谱技术对分离所得化合物进行结构鉴定。对分离得到化合物进行了抗枯草芽孢杆菌(Bacillus subtilis ATCC 6633)和抑制乙酰胆碱酯酶活性评价。共分离得到9个化合物,经鉴定为(E)-prop-1-enyl-2-hydroxypropanoate(1)、cyclo-(R-Pro-R-Phe)(2)、cyclo-(L-Val-L-Leu)(3)、cyclo-(L-Phe-L-Val)(4)、cyclo-(L-Leu-L-Phe)(5)、lumichrome(6)、2-(4-hydroxyphenyl)ethylacetate(7)、4-hydroxysattabacin(8)、3-hydroxybutan-2-yl 2-phenylacetate(9),其中化合物1为新天然产物。化合物5、6、8、9对Bacillus subtilis ATCC 6633有一定的抑制活性,化合物4、6对乙酰胆碱酯酶有较弱的抑制活性。The secondary metabolites of marine actinomycete Actinoplanes sp.M4I6 were isolated and identified,moreover their biological activities were evaluated.The compounds were separated and purified by Silica gel,C18,Sephadex LH-20 column chromatography and high performance liquid chromatography(HPLC),and their structures were identified by NMR spectroscopic method.These compounds were evaluated for anti-Bacillus subtilis(ATCC 6633)and inhibition of acetylcholinesterase activity.Nine compounds were separated and identified as(E)-prop-1-enyl-2-hydroxypropanoate(1),cyclo-(R-Pro-R-Phe)(2),cyclo-(L-Val-L-Leu)(3),cyclo-(L-Phe-L-Val)(4),cyclo-(L-Leu-L-Phe)(5),lumichrome(6),2-(4-hydroxyphenyl)ethylacetate(7),4-hydroxysattabacin(8),3-hydroxybutan-2-yl 2-phenylacetate(9),compound 1 is a new natural product.Compounds 5,6,8 and 9 showed certain inhibitory activities against Bacillus subtilis(ATCC 6633).Compounds 4 and 6 showed weak inhibitory activities against acetylcholinesterase.

关 键 词：海洋放线菌 游动放线菌属 化学成分 乙酰胆碱酯酶抑制活性 抗枯草芽孢杆菌活性 

分 类 号：P734.5[天文地球—海洋化学]