低盐胁迫下假微型海链藻脂肪酸代谢的转录组学分析  

Transcriptomic analysis of fatty acid metabolism in the Thalassiosira pseudonana under low salinity stress

作　　者：孙文杰[1,2] 李佳岷 王华龙[3] 米铁柱[1,2,4] 甄毓 SUN Wenjie;LI Jiamin;WANG Hualong;MI Tiezhu;ZHEN Yu(College of Environmental Science and Engineering,Ocean University of China,Qingdao 266100,China;Key Laboratory of Marine Environment and Ecology Ministry of Education,Ocean University of China,Qingdao 266100,China;College of Marine Life Science,Ocean University of China,Qingdao 266003,China;Laboratory for Marine Ecology and Environmental Science Qingdao National Laboratory for Marine Science and Technology,Qingdao 266071,China)

机构地区：[1]中国海洋大学环境科学与工程学院,山东青岛266100 [2]中国海洋大学海洋环境与生态教育部重点实验室,山东青岛266100 [3]中国海洋大学海洋生命学院,山东青岛266003 [4]青岛海洋科学与技术国家实验室海洋生态与环境科学功能实验室,山东青岛266071

出　　处：《热带海洋学报》2023年第5期92-103,共12页Journal of Tropical Oceanography

基　　金：青岛海洋科学与技术试点国家实验室“十四五”重大项目(2021QNLM040001);国家自然科学基金项目(41976133)。

摘　　要：文章以假微型海链藻(Thalassiosira pseudonana)转录组测序数据为基础,对差异表达的功能基因进行GO(gene ontology)和KEGG(kyoto encyclopedia of genes and genomes)富集分析,以研究低盐胁迫条件对其脂肪酸代谢通路相关功能基因表达的影响。结果发现,不同盐度培养下的假微型海链藻在生长期的第2天与第4天时细胞中与脂肪酸生物合成、脂肪酸延伸的相关基因(如:ACC1、arm)的表达量相对于生长第1天均显著升高,与脂肪酸降解相关基因(如:ACADM、ECI1)的表达量无显著变化;与脂肪酸的生物合成、延长相关基因的表达量在试验组和对照组间存在差异(如:起重要作用的KASⅠ、ACAA2等基因表达量显著增加,但也有少数处于下降或者微变动的状态),与脂肪酸降解过程相关基因的表达量显著上升。The expression of fatty acid metabolism-related genes of Thalassiosira pseudonana under different salinities and different growth stages was analyzed by transcriptomic sequencing in this study.A total of 40 coding genes that are related to the fatty acid metabolisms were retrieved from this study with distinct expression levels at different salinity concentrations.The results showed that the expression levels of genes related to fatty acid biosynthesis and elongation(such as ACC1,armB)in T.pseudonana cells were significantly higher at the second and fourth days compared to those at the first day across the salinity gradients,while the expression levels of genes related to fatty acid degradation(such as ACADM,ECI1)did not change significantly.The expression of genes related to fatty acid biosynthesis and elongation in the experimental group were differentially expressed compared to the control group(for example,the expression levels of KASⅠ,ACAA2 and other genes,which play an important role,increased significantly,but a few of them decreased or changed slightly),although the fatty acid degradation related genes were significantly increased in the experimental group.This study improves our understanding of the survival and adaptation strategies of diatoms in the offshore euryhaline environments,and supports the exploration of outbreak and vanishment of harmful algal blooms.

关 键 词：假微型海链藻 脂肪酸代谢 转录组 盐胁迫 

分 类 号：P735.2[天文地球—海洋生物学]