海洋本草软珊瑚共附生曲霉属真菌EGF7-0-1和EGF15-0-3共培养中甾体类成分研究Ⅱ  

Research on the steroids from the coculture of soft coral-associated fungi Aspergillus sp.EGF7-0-1 and EGF15-0-3

作　　者：司徒美霞 雷祖发 杨倩茹 邓胜毅 吴科键 陈鸿皓 韦霞 樊浩 张伟[2] 张翠仙[1] SITU Meixia;LEI Zufa;YANG Qianru;DENG Shengyi;WU Kejian;CHEN Honghao;WEI Xia;FAN Hao;ZHANG Wei;ZHANG Cuixian(School of Pharmaceutical Sciences,Guangzhou University of Chinese Medicine,Guangzhou 510006,China;The Middle School Attached to Sun Yat-sen University,Guangzhou 510275,China;Pharmaceutical college,Guangxi Medicinal University,Nanning 530021,China)

机构地区：[1]广州中医药大学,广东广州510006 [2]中山大学附属中学,广东广州510275 [3]广西医科大学,广西南宁530021

出　　处：《热带海洋学报》2023年第5期161-170,共10页Journal of Tropical Oceanography

基　　金：广东省自然资源厅省级促进经济高质量发展专项资金(海洋经济发展用途)项目(GDNRC[2021]48、GDNRC[2020]039);国家自然科学基金项目(82273845、81741160);广州市青少年科技教育项目(KP市2022027)。

摘　　要：文章模拟微生物自然生长环境,对两株海洋本草软珊瑚曲霉属真菌Aspergillus sp.EGF7-0-1和Aspergillus sp.EGF15-0-3进行共培养,以期发现新颖骨架成分。采用硅胶、反相色谱、羟丙基葡聚糖凝胶和高效液相色谱等方法对其次生代谢产物进行分离、纯化;采用高分辨质谱、核磁共振、旋光及物理常数对照等方法对所分离得到的化合物进行结构鉴定;采用全球天然产物分子网络(Global Natural Products Social Molecular Networking,GNPS)方法对次生代谢产物进行分析。从两株软珊瑚来源的曲霉属真菌大米共培养中分离得到10个甾体类化合物:Chaxine(1)、Herbarulide(2)、dankasterones A(3)、25-hydroxyergosta-4,6,8(14),22-tetraen3-one(4)、Ganodermasides D(5)、Ergosta-4,6,8(14),22-tetraen-3-one(6)、Ergosta-4,7,22-trien-3-one(7)、Isocyathisterol(8)、Stigmasta-4,22-dien-3-one(9)和β-sitostenone(10)。化合物1~3均为高度氧化重排的甾体类化合物。化合物4、5、7和9首次从曲霉属真菌中分离得到。GNPS分析表明与菌株EGF7-0-1和EGF15-0-3单培养相比,两株真菌共培养能诱导出更为丰富的甾体类化合物。In order to simulate the growth environment of microorganisms to change or increase the production of secondary metabolites in the presence of other microorganisms and protect the ecological environment of marine herbs,soft coral-associated two fungi Aspergillus sp.EGF7-0-1 and EGF15-0-3 were cocultured on rice medium.Firstly,GNPS(Global Natural Products Social Molecular Networking)method was used to analyze secondary metabolites.Then,the secondary metabolites were separated and purified by silica gel,ODS(Octadecylsilyl),Sephadex LH-20 and HPLC(Preparative High Performance Liquid Chromatography).Their structures were identified by HR-ESI-MS(High Resolution Electrospray Ionization Mass Spectroscopy),NMR(Nuclear Magnetic Resonance),ORD(Optical Rotatory Dispersion)and the relative physical constants were compared with the literature.Ten steroids were obtained and identified as Chaxine C(1),Herbarulide(2),dankasterones A(3),25-hydroxyergosta-4,6,8(14),22-tetraen3-one(4),Ganodermasides D(5),Ergosta-4,6,8(14),22-tetraen-3-one(6),Ergosta-4,7,22-trien-3-one(7),Isocyathisterol(8),Stigmasta-4,22-dien-3-one(9)andβ-sitostenone(10).Compounds 1~3 were steroids with oxidative rearrangement and 4,5,7 and 9 were firstly acquired from Aspergillus sp.compared with the single culture of EGF7-0-1 and EGF15-0-3.Based on the coculture strategy,the content types of steroids were more abundant.

关 键 词：海洋本草软珊瑚 共附生曲霉属真菌 共培养 甾体 

分 类 号：P745.3[天文地球—海洋生物学]