海洋本草软珊瑚共附生真菌Aspergillus sp.EGF7-0-1中酚酸类化学成分研究(Ⅱ)  

Phenolic acids from soft coral-associated symbiotic and epiphytic fungi Aspergillus sp.EGF7-0-1(Ⅱ)

作　　者：樊浩 何嘉鸿 韦霞 陈乐怡 陈欣琪 张翠仙[1] FAN Hao;HE Jiahong;WEI Xia;CHEN Leyi;CHEN Xinqi;ZHANG Cuixain(School of Pharmaceutical Sciences,Guangzhou University of Chinese Medicine,Guangzhou 510006,China;Pharmaceutical college,Guangxi Medicinal University,Nanning 530021,China)

机构地区：[1]广州中医药大学中药学院,广东广州510006 [2]广西医科大学药学院,广西南宁530021

出　　处：《热带海洋学报》2023年第5期171-177,共7页Journal of Tropical Oceanography

基　　金：广东省自然资源厅专项(GDNRC[2021]048、GDNRC[2020]039);国家自然科学基金项目(82273845、81741160);广东省大学生创新创业项目(S202010572122)。

摘　　要：为寻找海洋来源结构新颖的次生代谢产物,采用大米培养基对海洋本草软珊瑚(Sinularia sp.)共附生真菌Aspergillus sp.EGF7-0-1进行规模发酵,利用高效液相色谱等多种柱色谱技术对其乙酸乙酯提取物进行分离纯化;采用核磁共振波谱、高分辨质谱、旋光光谱等现代光谱学方法,并结合文献数据比对等对其进行结构鉴定,获得10个简单芳香酚酸化合物,分别为4-hydroxy-3-(3'-methyl-2'-butenyl)phenylacetic acid(1)、asperulosin C(2)、对羟基苯乙酸(3)、对羟基苯乙酸甲酯(4)、4-hydroxy-3-prenyl-benzoic acid(5)、4-hydroxy-3-(3-methylbut-2-enyl)benzaldehyde(6)、对羟基苯甲醛(7)、对羟基苯甲酸甲酯(8)、间羟基苯乙酸(9)和2,2'-oxybis(1,4)-ditert-butylbenzene(10),其中化合物1是新发现的天然产物。抗肿瘤活性研究表明,化合物1~10对人结肠癌细胞、人乳腺癌细胞、人肝癌细胞、小鼠肝癌细胞、小鼠乳腺癌细胞、小鼠黑色素瘤细胞和小鼠皮肤黑色素瘤细胞等肿瘤细胞均无活性。In order to discover the novel and bioactive secondary metabolites,the soft coral-associated(Sinularia sp.)symbiotic and epiphytic fungi Aspergillus sp.EGF7-0-1 was fermented by using rice medium.The metabolites were isolated by the chromatography technology and high performance liquid chromatography(HPLC).Ten phenolic acids compounds were obtained.According to the 1D and 2D nuclear magnetic resonance(NMR),high resolution electrospray ionization mass spectroscopy(HR-ESI-MS),optical rotatory dispersion(ORD),and comparison with the literature,their structures were determined as 4-hydroxy-3-(3'-methyl-2'-butenyl)phenylacetic acid(1),asperulosin C(2),4-hydroxyphenylacetic acid(3),methyl 4-hydroxyphenylacetate(4),4-hydroxy-3-prenyl-benzoic acid(5),4-hydroxy-3-(3-methylbut-2-enyl)benzaldehyde(6),p-Hydroxybenzaldehyde(7),methyl 4-hydroxybenzoate(8),3-hydroxyphenylacetic acid(9)and 2,2'-oxybis(1,4)-ditert-butylbenzene(10).Among them,compound 1 was a new natural product.Antitumor activity studies showed that compounds 1~10 were not active against HCT116,MCF7,Huh 7,Hepa 1-6,4T1,B16 and F10 tumor cells.

关 键 词：海洋本草软珊瑚 Aspergillus sp.EGF7-0-1 酚酸类 新天然产物 结构鉴定 

分 类 号：P745.3[天文地球—海洋生物学]