锯缘青蟹产组胺菌的分离与鉴定  

Isolation and Identification of Histamine-Producing Bacteria from Scylla serrata

作　　者：陈德强 谢吉红 孙菲霞 黄丽 CHEN Deqiang;XIE Jihong;SUN Feixia;HUANG Li(Beibu Gulf University,Qinzhou 535011,China;Guangxi College and Universities Key Laboratory of High-value Utilization of Seafood and Prepared Food in Beibu Gulf,Qinzhou 535011,China)

机构地区：[1]北部湾大学,广西钦州535011 [2]广西高校北部湾海产品高值化利用与预制食品重点实验室,广西钦州535011

出　　处：《现代食品》2023年第15期171-173,177,共4页Modern Food

基　　金：北部湾大学大学生创新创业训练计划项目(S202111607041);广西科技计划项目(桂科AD19245119);北部湾大学引进高层次人才科研启动项目(2019KYQD11、2019KYQD12)。

摘　　要：本研究从锯缘青蟹中分离得到24株产组胺菌。经检测,24株产组胺菌均携带组氨酸脱羧酶hdc基因,通过16S rDNA比对分析,共鉴定为9个属15个细菌种,其中Acinetobacter baumannii为优势分离菌种,占比为25.0%;产组胺能力检测表明,24株产组胺菌发酵上清液平均组胺含量为33.97 mg·L^(-1),Aeromonas hydrophilaH_(20)的组胺含量最高达45.06 mg·L^(-1)。本研究为进一步了解锯缘青蟹产组胺微生物种类,以及加强锯缘青蟹产品质量安全控制提供一定参考。In this study,24 strains of histamine producing bacteria were isolated from Scylla serrata.All these 24 bacterial strains carried the histidine decarboxylase gene hdc.A total of 9 genera and 15 bacterial species were identified through 16S rDNA analysis,among which Acinetobacter baumannii was the dominant isolated strain,accounting for 25.0%.The average histamine content in the fermentation supernatant of the 24 strains was 33.97 mg·L^(-1),with the highest histamine content 45.06 mg·L^(-1)in Aeromonas hydrophilaH_(20).This study provides some reference for further comprehension of the types of histamine producing microorganisms and the quality and safety control in Scylla serrata.

关 键 词：锯缘青蟹 产组胺菌 分离鉴定 

分 类 号：F762.6[经济管理—产业经济]