20种肿瘤化疗药物对Treg细胞增殖的影响  

Effects of twenty kinds of chemotherapeutic agents on the proliferation of Treg cells

作　　者：黄薇 凌睿云 高乐欣 唐文 钱令波 陈峰阳 HUANG Wei;LING Ruiyun;GAO Lexin;TANG Wen;QIAN Lingbo;CHEN Fengyang(School of Basic Medical Sciences&Forensic Medicine,Hangzhou Medical College,Hangzhou 310053,China)

机构地区：[1]杭州医学院基础医学与法医学院,浙江杭州310053

出　　处：《基础医学与临床》2023年第10期1518-1521,共4页Basic and Clinical Medicine

基　　金：健康浙江专项计划(YS2022003);国家级大学生创新创业训练计划(202213023018);浙江省大学生创新创业训练计划(S202213023054)。

摘　　要：目的观察20种肿瘤化疗药物对调节性T细胞(Treg)增殖的影响。方法选取20种常见的肿瘤化学治疗(化疗)药物,采用IL-2诱导Treg细胞增殖,通过CellTrace标记和流式细胞测量术测定。结果用12种化疗药在无细胞毒性的浓度下作用后,CD4^(+)T细胞中Foxp3^(+)Treg细胞比例明显减少,分裂的Treg细胞数明显降低(P<0.05)。结论12种化疗药物,其中包括3个影响微管蛋白药物、3个干扰转录和RNA合成的药物及2个拓扑异构酶抑制剂能显著抑制Treg细胞的增殖。Objective To observe the effects of 20 chemotherapeutic agents on the proliferation of Treg cells.Methods Twenty agents used in routine chemotherapy were selected and assayed on IL-2-induced Treg cell proliferation through CellTrace labeling and flow cytometry.Results After treatment with 12 chemotherapeutic agents at non-cytotoxic concentration,the proportion as well as the number of Foxp3^(+)Treg cells in CD4^(+)T cells were significantly reduced.Conclusions Twelve chemotherapeutic agents including 3 tubulin interfering agents,3 transcription and RNA synthesis interfering agents and 2 topoisomerase inhibitors inhibited the proliferation of Treg cells.

关 键 词：肿瘤免疫 化学治疗 TREG细胞 

分 类 号：R979.1[医药卫生—药品]