鮰爱德华菌密度感应系统LuxI/LuxR的克隆及生物信息学分析  

Molecular Cloning and Bioinformatics Analysis of Quorum Sensing System LuxI/LuxR of Edwardsiella ictaluri

作　　者：郭锦志 马士杰 韩先干[2] 付立霞[1] GUO Jin-zhi;MA Shi-jie;HAN Xian-gan(Yangzhou University College of Animal Science and Technology,Yangzhou,Jiangsu 225009;Shanghai Veterinary Research Institute,Chinese Academy of Agricultural Sciences,Shanghai 200241)

机构地区：[1]扬州大学动物科学与技术学院,江苏扬州225009 [2]中国农业科学院上海兽医研究所,上海200241

出　　处：《安徽农业科学》2023年第18期107-114,共8页Journal of Anhui Agricultural Sciences

基　　金：农业农村部渔用药物创制重点实验室和广东省水产动物免疫技术重点实验室项目(201801)。

摘　　要：克隆鮰爱德华菌(Edwardsiella ictaluri)YCH株酰基高丝氨酸内酯(Acyl homoserine lactones, AHLs)合成酶(LuxI)和AHLs受体蛋白(LuxR)的全长基因,并对其进行生物信息学分析。序列分析结果显示,LuxI全长666 bp,共编码221个氨基酸;LuxR全长720 bp,编码239个氨基酸。构建LuxI/LuxR的系统发生树,结果显示,鮰爱德华菌LuxI/LuxR与迟缓爱德华菌(Edwardsiella tarda)、杀鱼爱德华菌(Edwardsiella piscicida)、保科爱德华菌(Edwardsiella hoshinae)等有较近亲缘关系。利用SWISS-Model软件,分别以1K4J.1.A(46.15%)和5l09.1.A(40.83%)蛋白作为模板对LuxI蛋白的8-189区域和LuxR蛋白2个功能结构域Pfam:Autoind_bind(9~151氨基酸)和HTH_LUXR(171~228氨基酸)进行分子模拟,其中LuxI结构域内有明显的“V”形疏水腔结构,LuxR的N-端结构域有α-β-α排列,C-端结构域内有螺旋—转角—螺旋结构。The full-length genes of Acyl homoserine lactones(AHLs)synthase(LuxI)and AHLs receptor protein(LuxR)of YCH strain of Edwardsiella ictaluri were cloned and bioinformatics was performed.Sequence analysis showed that LuxI was 666 bp in length and encoded 221 amino acids.LuxR is 720 bp in length and encodes 239 amino acids.The phylogenetic tree of LuxI/LuxR was constructed,and the results showed that LuxI/LuxR of Edwardsiella ictaluri is closely related to Edwardsiella tarda,Edwardsiella piscicida,Edwardsiella hoshinae,etc.Using SWISS-Model software,1K4J.1.A(46.15%)and 5L09.1.A(40.83%)were used as templates for molecular simulation of LuxI protein 8-189 region and LuxR protein Pfam:Autoind_bind(9-151 amino acid)and HTH_LUXR(171-228 amino acid).The LuxI functional domain has obvious V-shaped hydrophobic cavity structure,the N-terminal functional domain of LuxR hasα-β-αarrangement,and the C-terminal functional domain has helix-rotation-helix structure.This paper lays a foundation for further research on the LuxI/LuxR-mediated,quorum sensing system of E.ictaluri,and also provides a reference for the prevention and treatment of diseases caused by it.

关 键 词：鮰爱德华菌 密度感应系统 LuxI/LuxR 基因克隆 生物信息学 

分 类 号：S941.42[农业科学—水产养殖]