缺氧损伤模型及抗缺氧药物筛选研究进展  

Research progress of hypoxic injury model and anti-hypoxic drug screening

作　　者：张思岳 张惠雯 夏青 王荣春 张云 刘可春 ZHANG Siyue;ZHANG Huiwen;XIA Qing;WANG Rongchun;ZHANG Yun;LIU Kechun(Biology Institute of Shandong Academy of Sciences,Qilu University of Technology(Shandong Academy of Sciences),Jinan 250103,China;Engineering Research Center of Zebrafish Models for Human Diseases and Drug Screening of Shandong Province,Jinan 250103,China)

机构地区：[1]齐鲁工业大学(山东省科学院),山东省科学院生物研究所,山东济南250103 [2]山东省人类疾病斑马鱼模型与药物筛选工程技术研究中心,山东济南250103

出　　处：《药物评价研究》2023年第9期2037-2045,共9页Drug Evaluation Research

基　　金：泰山学者工程资助项目(tsqn202211204);山东省优秀青年人才基金项目(ZR2020YQ60);济南市“高校20条”资助项目(2021GXRC047,2020GXRC053)。

摘　　要：在临床上,缺氧是引起机体损伤甚至导致机体死亡的最常见因素,因此建立了一系列缺氧损伤模型,如体外细胞模型上,通过液态石蜡封闭法、厌氧袋产气法、混合气体培养法等方法建立缺氧损伤模型。在动物模型(如大、小鼠和斑马鱼模型)上,通过混合气体培养法、钠石灰吸收法、动脉结扎法以及化学试剂处理建立缺氧损伤模型;并通过检测缺氧后存活时间、酶学指标、血气指标、组织病理切片及缺氧相关因子的变化情况进行抗缺氧药物的筛选和评价。综述缺氧损伤模型及抗缺氧药物筛选的研究进展,以期为临床治疗缺氧提供理论依据和参考。In clinical practice,hypoxia is the most common factor that causes organism injury or even leads to organism death.Therefore,research workers have established a series of hypoxic injury models,such as in vitro cellular models,by liquid paraffin closure method,anaerobic bag gas production method,mixed gas culture method and other methods to establish hypoxic injury models.On animal models such as rat and mouse and zebrafish models,hypoxic injury models were established by mixed gas incubation method,sodium-lime absorption method,arterial ligation method and chemical reagent treatment.And the anti-hypoxic drugs were screened and evaluated by detecting the survival time,enzymatic indexes,blood gas indexes,histopathological sections and changes of hypoxia-related factors after hypoxia.Research progress of hypoxic injury model and anti-hypoxic drug screening were summarized,in order to provide theoretical basis and reference for clinical treatment of hypoxia.

关 键 词：缺氧损伤 细胞模型 动物模型 活性评价指标 抗缺氧药物 药物筛选 

分 类 号：R965[医药卫生—药理学]