TGF-β1/Smad3信号通路与弓形虫感染孕妇不良妊娠结局的关系  

Relationship between TGF-β1/Smad3 signaling pathway and adverse pregnancy outcomes caused by Toxoplasma gondii infection

作　　者：姜姗姗 杨洪艳[1] 金男 李烁 马纳新[1] JIANG Shan-shan;YANG Hong-yan;JIN Nan;LI Shuo;MA Na-xin(Shengjing Hospital of China Medical University,Shenyang,Liaoning 110023,China)

机构地区：[1]中国医科大学附属盛京医院第五产科病房,辽宁沈阳110023

出　　处：《中华医院感染学杂志》2023年第13期2042-2046,共5页Chinese Journal of Nosocomiology

基　　金：辽宁省科研基金资助项目(201921411)。

摘　　要：目的探究转化生长因子β1(TGF-β1)β1/Smad3信号通路与弓形虫感染孕妇不良妊娠结局的关系。方法选择2019年10月-2020年10月中国医科大学附属盛京医院就诊的98例孕妇,其中弓形虫感染32例,无弓形虫感染66例,收集脐血、胎膜组织,采用聚合酶链式反应检测TGF-β1、Smad3、叉头样转录因子3(Foxp3)mRNA,采用流式细胞仪检测脐血CD4+CD25+Foxp3+调节性T细胞(Treg)细胞比例,采用蛋白印迹法检测胎盘TGF-β1、磷酸化Smad3(P-Smad3)、Foxp3蛋白表达和酶联免疫吸附法检测血清白细胞介素-10(IL-10)、干扰素-γ(IFN-γ)水平,计算IFN-γ/IL-10,比较各组检测结果,分析检测指标间的关系。结果感染组孕妇孕周短于对照组(P<0.05),新生儿体质量、胎盘质量低于对照组(P<0.05),不良妊娠结局发生率高于对照组(P<0.05);感染组孕妇胎盘中TGF-β1、P-Smad3、Foxp3的mRNA及其蛋白表达水平、脐血CD_(4)^(+)CD_(25)^(+)Foxp^(3+)Treg细胞比例、IL-10均低于对照组(P<0.05),血清IFN-γ、IL-10/IFN-γ水平高于对照组(P<0.05);弓形虫感染孕妇脐血CD_(4)^(+)CD_(25)^(+)Foxp^(3+)Treg细胞比例与胎盘TGF-β1、P-Smad3、Foxp3的mRNA及蛋白表达水平正相关(P<0.05),与血清IFN-γ/IL-10水平负相关(P<0.05)。结论弓形虫感染可致孕妇TGF-β1/Smad3信号通路因子水平降低和Th1/Th2失衡,打破孕妇维持正常妊娠的免疫耐受状态,增加不良妊娠结局风险。OBJECTIVE To explore the relationship between transforming growth factor-β1(TGF-β1)/Smad3 signaling pathway and adverse pregnancy outcomes of the pregnant women with Toroplasma gondii infection.METHODS A total of 98 pregnant women who were treated in Shengjing Hospital of China Medical University from Oct 2019 to Oct 2020 were enrolled in the study,32 of whom had T.gondii infection,and 66 did not have T.gondii infection.The umbilical cord blood specimens and fetal membrane tissues were collected;the expressions of TGF-βl,Smad3 and forkhead box P3(Foxp3)mRNA were detected by polymerase chain reaction,the percentage of CD+CDas+Foxp3+regulatory T cell(Treg)cellin umbilical cord blood was detected by flow cytometry,the expressions of TGF-β1,phosphorylated Smad3(P-Smad3)and Foxp3 proteins in placenta were detected by Western blot assay,the levels of serum interleukin-10(IL-10)and interferon-(IFN-)were detected by enzyme-linked immunosorbent assay,the IFN-/IL-10 was calculated,the levels of above indexes were compared among the groups,and the relationship among the indexes was observed.RESULTS The gestational weeks of the infection group were significantly shorter than those of the control group(P<o.05),the neonatal body weight and placenta of the infection group were significantly lower than those of the control group(P<0.05);the incidence of adverse pregnancy outcomes of the infection group was significantly higher than that of the control group(P<0.05).The expression levels of TGF-βl,P-Smad3,Foxp3 mRNA and their proteins in placenta tissues,percentage of CD_(4)^(+)CD_(25)^(+)Foxp^(3+)Treg cell in umbilical cord blood and IL-10 were significantly lower in the infection group than in the control group(P<0.05).The levels of serum IFN-and IL-10/IFN-of the infection group were significantly higher than those of the control group(P<0.05).The percentage of CD_(4)^(+)CD_(25)^(+)Foxp^(3+)Treg cell in umbilical cord blood of the pregnant women with T.gondii infection was positively correlated with the expression levels o

关 键 词：弓形虫感染 孕妇 不良妊娠结局 转化生长因子β1/Smad3信号通路 免疫耐受 

分 类 号：R531.8[医药卫生—内科学]