甘草地上部分化学成分的分离与鉴定  

Isolation and identification of chemical constituents from aerial parts of Glycyrrhiza uralensis

作　　者：常冠华 张鲁 崔洁[3] 王文全[2,3,4] 侯俊玲[2,4] CHANG Guan-hua;ZHANG Lu;CUI Jie;WANG Wen-quan;HOU Jun-ling(Beijing Wehand-Bio Pharmaceutical Co.,Ltd.,Beijing 102629,China;School of Chinese Materia Medica,Beijing University of Chinese Medicine,Beijing 102488,China;Institute of Medicinal Plant Development,Chinese Academy of Medicinal Sciences and Peking Union Medical College,Beijing 100193,China;Engineering Research Center of Good Agricultural Practice for Chinese Crude Drugs,Ministry of Education,Beijing 100102,China)

机构地区：[1]北京五和博澳药业股份有限公司,北京102629 [2]北京中医药大学中药学院,北京102488 [3]中国医学科学院北京协和医学院药用植物研究所,北京100193 [4]中药材规范化生产教育部工程研究中心,北京100102

出　　处：《中国中药杂志》2023年第16期4413-4420,共8页China Journal of Chinese Materia Medica

基　　金：国家重点研发计划项目(2018YFC1706500)。

摘　　要：研究甘草Glycyrrhiza uralensis地上部分的化学成分。将甘草地上部分醇提物采用大孔树脂、硅胶、凝胶等不同柱色谱技术,并经制备液相及重结晶等进行分离纯化,共分离得到13个单体化合物,分别鉴定为(2S)-6-[(Z)-3-羟甲基-2-丁烯基]-5,7,3′-三羟基-4′-甲氧基-二氢黄酮(1)、(2S)-8-[(E)-3-羟甲基-2-丁烯基]-5,7,3′,5′-四羟基-二氢黄酮(2)、α,α′-二氢-5,4′-二羟基-3-乙酰氧基-2-异戊烯基茋(3)、6-异戊烯基槲皮素(4)、6-异戊烯基槲皮素-3-甲醚(5)、刺芒柄花素(6)、槲皮素-3,3′-二甲醚(7)、金圣草素(8)、香叶木素(9)、(10E,12Z,14E)-9,16-二羰基-10,12,14-三烯-十八碳酸(10),5,7,3′,4′-四羟基-6-异戊烯基-二氢黄酮(11)、柚皮素(12)、邻苯二甲酸二丁酯(13)。其中化合物1~3为未见报道的新化合物,化合物10和13为首次在甘草地上部分被分离鉴定。The present study investigated the chemical constituents from the aerial parts of Glycyrrhiza uralensis.The ethanol extract of the aerial parts of G.uralensis was separated and purified by different column chromatographies such as macroporous resin,silica gel,and Sephadex LH-20,and through preparative HPLC and recrystallization.Thirteen compounds were isolated and identified as(2S)-6-[(Z)-3-hydroxymethyl-2-butenyl]-5,7,3′-trihydroxy-4′-methoxy-dihydroflavanone(1),(2S)-8-[(E)-3-hydroxymethyl2-butenyl]-5,7,3′,5′-tetrahydroxy-dihydroflavanone(2),α,α′-dihydro-5,4′-dihydroxy-3-acetoxy-2-isopentenylstilbene(3),6-prenylquercetin(4),6-prenylquercetin-3-methyl ether(5),formononetin(6),3,3′-dimethylquercetin(7),chrysoeriol(8),diosmetin(9),(10E,12Z,14E)-9,16-dioxooctadec-10,12,14-trienoic acid(10),5,7,3′,4′-tetrahydroxy-6-prenyl-dihydroflavanone(11),naringenin(12),dibutylphthalate(13).Compounds 1-3 are new compounds,and compounds 10 and 13 are isolated from aerial parts of this plant for the first time.

关 键 词：甘草 化学成分 分离鉴定 黄酮类成分 

分 类 号：R284.1[医药卫生—中药学]