六味地黄汤对碘酸钠诱导的干性年龄相关性黄斑变性大鼠视网膜组织色素上皮衍生因子的表达及氧化应激水平的影响  

Effect of Liuwei Dihuang Decoction on Expression of Pigment Epithelium-derived Factors and Oxidative Stress in Retinal Tissue of Rats with Dry Age-related Macular Degeneration Induced by Sodium Iodate

作　　者：张琦[1] 赵磊[2] 左韬[2] ZHANG Qi;ZHAO Lei;ZUO Tao(Liaoning University of Traditional Chinese Medicine,Shenyang Liaoning 110032;The Second Affiliated Hospital of Liaoning University of Traditional Chinese Medicine,Shenyang Liaoning 110000)

机构地区：[1]辽宁中医药大学,辽宁沈阳110032 [2]辽宁中医药大学附属第二医院,辽宁沈阳110000

出　　处：《世界中西医结合杂志》2023年第8期1494-1499,共6页World Journal of Integrated Traditional and Western Medicine

基　　金：国家中医药管理局“中医药循证能力建设项目”(2019XZZX-YK008);第一批辽宁省老中医药专家学术经验继承工作项目[辽中医药函(2021)1号]。

摘　　要：目的 观察六味地黄汤对碘酸钠(NaIO3)腹腔注射致干性年龄相关性黄斑变性(Dry AMD,dAMD)模型大鼠视网膜结构及色素上皮衍生因子(Pigment Epithelium-derived Factor,PEDF)、活性氧(Reactiveoxygenspecies,ROS)、丙二醛(Malonicdialdehyde,MDA)和还原型谷胱甘肽(Glutathione,GSH)表达水平的影响,探讨六味地黄汤对dAMD模型大鼠视网膜保护作用的机制。方法 将45只SPF级雌性SD大鼠采用随机数字表法分为空白组15只和dAMD组30只,空白组腹腔注射生理盐水(60 mg/kg),dAMD组腹腔注射NaIO3(60 mg/kg)。再将dAMD组随机分为模型组和中药组,每组各15只。空白组、模型组灌服生理盐水6.75 g/(kg·d),中药组灌服六味地黄汤6.75 g/(kg·d),连续14 d。末次给药后2 h时,通过眼底彩照观察各组大鼠眼底情况后取材,通过HE染色观察各组大鼠视网膜病理改变情况;通过免疫组化法、Western-blot法检测各组大鼠视网膜PEDF表达情况;通过Elisa法检测各组大鼠视网膜GSH、MDA、ROS的表达水平。结果 dAMD模型大鼠视网膜组织中MDA、ROS表达程度增高,差异有统计学意义(P<0.05);PEDF、GSH表达程度降低,差异有统计学意义(P<0.05)。六味地黄汤通过降低MDA、ROS表达水平、提高PEDF、GSH表达水平而达到保护视网膜的作用。结论 六味地黄汤对dAMD模型大鼠视网膜具有保护作用,其机制可能与调控PEDF及氧化应激水平相关。Objective To observe the effects of Liuwei Dihuang Decoction on retinal structure and the expression levels of pigment epithelium-derived factor(PEDF),reactive oxygen species(ROS),malondialdehyde(MDA),and glutathione(GSH)in a sodium iodate(NaIO3)-induced dry age-related macular degeneration(dAMD)rat model and explore the mechanisms underlying the protective effects of Liuwei Dihuang Decoction on the retina in the dAMD model.Methods Forty-five SPF female Sprague-Dawley rats were randomly divided into a blank group(n=15)and a dAMD group(n=30).The blank group received an intraperitoneal injection of normal saline(60 mg/kg),while the dAMD group received an intraperitoneal injection of NaIO3(60 mg/kg).The dAMD group was further divided into a model group and a Chinese medicine group,with 15 rats in each group.The blank group and the model group were orally administered normal saline(6.75 g/kg·d),and the Chinese medicine group was orally administered Liuwei Dihuang Decoction 6.75 g/(kg·d)for 14 consecutive days.Two hours after the last administration,retinal samples were collected after fundus photography.Hematoxylin and eosin(HE)staining was performed to observe retinal pathological changes.Immunohistochemistry and Western blot were used to detect the expression of PEDF in the retina of each group.ELISA was performed to detect the expression levels of GSH,MDA,and ROS in the retina of each group.Results In the dAMD model rats,the expression levels of MDA and ROS in the retinal tissue increased significantly(P<0.05),while the expression levels of PEDF and GSH decreased significantly(P<0.05).Liuwei Dihuang Decoction protected the retina by reducing the expression levels of MDA and ROS and increasing the expression levels of PEDF and GSH.Conclusion Liuwei Dihuang Decoction has a protective effect on the retina of dAMD model rats,possibly by regulating PEDF and oxidative stress levels.

关 键 词：六味地黄汤 年龄相关性黄斑变性 视网膜 氧化应激 

分 类 号：R285.5[医药卫生—中药学]