针灸对气滞血瘀型慢性输卵管炎模型大鼠血流变及炎症因子的影响  

Effects of Acupuncture and Moxibustion on Blood Flow and Inflammatory Factors in Rats with Chronic Salpingitis of Qi Stagnation and Blood Stasis Syndrome

作　　者：吴林霖 刘丽[2] WU Lin-lin;LIU Li(Graduate School,Heilongjiang University of Chinese Medicine,Harbin Heilongjiang 150040;The Second Department of Gynecology,First Affiliated Hospital of Heilongjiang University of Chinese Medicine,Harbin Heilongjiang 150040)

机构地区：[1]黑龙江省中医药大学研究生院,黑龙江哈尔滨150040 [2]黑龙江中医药大学附属第一医院妇二科,黑龙江哈尔滨150040

出　　处：《世界中西医结合杂志》2023年第8期1500-1505,共6页World Journal of Integrated Traditional and Western Medicine

基　　金：黑龙江省自然科学基金面上项目(H2015026)。

摘　　要：目的 探讨针灸治疗气滞血瘀型慢性输卵管炎的作用机制。方法 将40只SPF级雌性SD大鼠,按随机数字表法随机分为空白组、模型组、手针组、电针组,每组各10只。除空白组外,其余大鼠建立慢性输卵管炎症模型,叠加气滞血瘀证候模型并复制,造模成功后,除模型组外,其余大鼠进行手针或电针干预28 d,取穴:“关元”“足三里”“三阴交”及“肾俞”。治疗结束后观察各组大鼠宏观表征,留取腹主动脉血及两侧输卵管组织,血清测定各组大鼠血流变学及白介素10(Interleukin-10,IL-10)、白介素17(Interleukin-17,IL-17)及I型胶原蛋白(Collagen 1,COL-1)mRNA水平,输卵管组织中COL-1表达,Masson染色输卵管组织切片。结果 气滞血瘀证候积分、血浆黏度、全血黏度3.0、血清IL-17、COL-1 mRNA水平及输卵管组织中COL-1表达,模型组均明显高于空白组,模型组血清IL-10明显低于空白组,差异均有统计学意义(P<0.01),Masson染色模型组大鼠蓝色胶原纤维沉积最多;针灸治疗有效降低模型大鼠气滞血瘀证候积分、血浆黏度、全血黏度30、血清IL-17、COL-1 mRNA及组织中COL-1表达,增加血清IL-10水平,有效减少了胶原纤维沉积;手针组、电针组组间比较:中医证候积分、血流变学、血清及组织中COL-1表达,差异无统计学意义(P>0.05),电针组血清IL-10表达高于手针组,血清IL-17低于手针组,差异有统计学意义(P<0.05)。结论 针灸治疗可以有效调节模型大鼠气滞血瘀证候、降低血液黏度、降低IL-17、COL-1表达、升高IL-10表达,减少输卵管组织胶原纤维沉积,对气滞血瘀型慢性输卵管炎的治疗起到积极作用。Objective To explore the mechanism of acupuncture and moxibustion treatment for chronic salpingitis of qi stagnation and blood stasis syndrome.Methods Forty female SPF-grade SD rats were randomly divided into a blank group,a model group,a hand acupuncture group,and an electroacupuncture(EA)group,with 10 rats in each group.The chronic salpingitis model of qi stagnation and blood stasis syndrome was induced in rats except for those in the blank group.After successful modeling,model rats except for those in the model group underwent hand acupuncture or EA intervention for 28 days at acupoints Guanyuan(CV 4),Zusanli(ST 36),Sanyinjiao(SP 6),and Shenshu(BL 23).After treatment,the macroscopic characteristics of rats in each group were observed,and abdominal aortic blood and bilateral fallopian tube tissues were collected.Hemorheological parameters and mRNA levels of interleukin-10(IL-10),interleukin-17(IL-17),and Collagen 1(COL-1)in rats were determined.COL-1 expression in fallopian tube tissues was assessed and fallopian tube tissues underwent Masson staining.Results Qi stagnation and blood stasis syndrome scores,plasma viscosity,whole-blood viscosity at 3.0,serum IL-10,IL-17,COL-1 mRNA levels,and COL-1 expression in fallopian tube tissues in the model group were significantly higher than those in the blank group(all P<0.01).Masson staining showed the most significant blue collagen fiber deposition in model rats.Acupuncture and moxibustion treatment effectively reduced the qi stagnation and blood stasis syndrome scores,plasma viscosity,whole-blood viscosity at 30,serum IL-10,IL-17,COL-1 mRNA levels,and COL-1 expression in fallopian tube tissues,resulting in reduced collagen fiber deposition.Comparison between the hand acupuncture group and the EA group showed no statistically significant differences in TCM syndrome scores,hemorheological parameters,and serum or tissue COL-1 expression(all P>0.05).Serum IL-10 expression was higher in the EA group than in the hand acupuncture group,while serum IL-17 expression was lower(

关 键 词：针灸 慢性输卵管炎 气滞血瘀 

分 类 号：R285.5[医药卫生—中药学]