载脂蛋白AⅡ基因-265T/C多态性与冠心病患者脂类代谢及冠脉狭窄程度相关性研究  

Correlation between apolipoprotein AⅡ gene 265T/C polymorphism and lipid metabolism and degree of coronary stenosis in patients with coronary heart disease

作　　者：金银浩 刘桂清 JIN Yinhao;LIU Guiqing(Graduate Student Training Base,the First Affiliated Hospital of Qiqihar Medical College,Jinzhou Medical University,Qiqihar 161041,China)

机构地区：[1]锦州医科大学齐齐哈尔医学院附属第一医院研究生培养基地,黑龙江齐齐哈尔161041 [2]齐齐哈尔医学院附属第一医院,黑龙江齐齐哈尔161041

出　　处：《陕西医学杂志》2023年第10期1331-1334,1339,共5页Shaanxi Medical Journal

基　　金：黑龙江省省属高等学校基本科研业务资助项目(2021-KYYWF-0339)。

摘　　要：目的:探究载脂蛋白AⅡ基因-265T/C(ApoAⅡ-265T/C)多态性与冠心病(CHD)患者脂类代谢及冠脉狭窄程度相关性。方法:选择96例CHD患者为研究组。另取同期健康体检者100例作为对照组。分别采集研究组入院后翌日清晨空腹静脉血以及对照组入院当天空腹静脉血。通过聚合酶链反应结合限制片段长度多态性(PCR-RFLP)实现对所有受试者ApoAⅡ-265T/C基因多态性的检测。并通过全自动生化分析仪以及相关试剂盒完成血清脂代谢指标水平的检测。此外,对CHD患者开展冠脉造影,将所有CHD患者根据Gensini评分的差异分为轻度狭窄组(≤20分)、中度狭窄组(>20分且≤45分)及重度狭窄组(>45分)。比较研究组和对照组ApoAⅡ-265T/C基因多态性、血清脂代谢指标水平。分析不同冠脉狭窄程度CHD患者ApoAⅡ-265T/C基因多态性、血清脂代谢指标水平。并通过多因素Logistic回归分析明确CHD患者冠脉中/重度狭窄的影响因素。结果:CHD组血清ApoAⅠ及ApoB水平均低于对照组(均P<0.05),ApoAⅡ-265T/C多态性CC+CT基因型频率、C等位基因型频率高于对照组,差异有统计学意义(均P<0.05)。中/重度狭窄组患者的CC+CT基因型频率、C等位基因频率均高于轻度狭窄组,差异有统计学意义(均P<0.05)。中/重度狭窄组血清ApoAⅠ及ApoB水平均低于轻度狭窄组,差异有统计学意义(均P<0.05)。经多因素Logistic回归分析发现,CC+CT基因型频率以及C等位基因型均是CHD患者冠脉中/重度狭窄的危险因素(均P<0.05)。结论:CHD患者存在ApoAⅡ-265T/C基因CC+CT基因型频率、C等位基因型异常,且和CHD患者中/重度狭窄密切相关。Objective:To investigate and analyze the correlation between apolipoprotein AⅡgene 265T/C(ApoAⅡ-265T/C)polymorphism and lipid metabolism and coronary artery stenosis degree in patients with coronary heart disease(CHD).Methods:A total of 96 patients with CHD were enrolled as study group.Another 100 healthy subjects were selected as the control group.Fasting venous blood was collected in the early morning of the next day after admission in the study group and in the control group.ApoAⅡ-265T/C gene polymorphism was detected in all subjects by polymerase chain reaction combined with restricted fragment length polymorphism(PCR-RFLP).The level of serum lipid metabolism index was detected by automatic biochemical analyzer and related kits.In addition,coronary angiography was performed for CHD patients,and all CHD patients were divided into mild stenosis group(≤20 points),moderate stenosis group(>20 points and≤45 points)and severe stenosis group(>45 points)according to Gensini scores.The levels of ApoAⅡ-265T/C gene polymorphism and serum lipid metabolism were compared between the study group and the control group.ApoAⅡ-265T/C gene polymorphism and serum lipid metabolism were analyzed in CHD patients with different degrees of coronary stenosis.Multivariate Logistic regression analysis was conducted to determine the influencing factors of moderate/severe coronary stenosis in CHD patients.Results:The levels of serum ApoAⅠand ApoB in CHD group were lower than those in control group,and ApoAⅡ-265T/C polymorphic CC+CT genotype frequency and C allele genotype frequency were higher than those in control group(all P<0.05).CC+CT genotype frequency and C allele frequency in moderate and severe stenosis group were higher than those in mild stenosis group(all P<0.05).The levels of serum ApoAⅠand ApoB in moderate and severe stenosis groups were lower than those in mild stenosis group(all P<0.05).Multivariate Logistic regression analysis showed that CC+CT genotype frequency and C allele were risk factors for moderat

关 键 词：冠心病 载脂蛋白AⅡ基因-265T/C 冠状动脉 GENSINI评分 基因多态性 狭窄 

分 类 号：R541.4[医药卫生—心血管疾病]