对暗黑鳃金龟幼虫高毒力的球孢白僵菌筛选及其模拟田间防效评价  

Screening and simulated field efficacy evaluation of Beauveria bassiana with high virulence to Holotrichia parallela(Coleoptera:Scarabaeidae)larvae

作　　者：朱长建 马雨梦 李斌 梁佳惠 曹付 杨洋[3] 刘苏[1] 李茂业[1] ZHU Chang-Jian;MA Yu-Meng;LI Bin;LIANG Jia-Hui;CAO Fu;YANG Yang;LIU Su;LI Mao-Ye(Key Laboratory of Biology and Sustainable Management of Plant Disease and Pests of Anhui Higher Education Institutes,School of Plant Protection,Anhui Agricultural University,Hefei 230026,China;Sichuan Provincial Branch of China National Tobacco Corporation,Chengdu 610041,China;Yibin Branch of Sichuan Provincial Tobacco Corporation,Yibin 644002,China)

机构地区：[1]安徽农业大学植物保护学院,植物病虫害生物学与绿色防控安徽普通高校重点实验室,合肥230036 [2]中国烟草总公司四川省公司,成都610041 [3]四川省烟草公司宜宾市公司,宜宾644002

出　　处：《昆虫学报》2023年第8期1009-1019,共11页Acta Entomologica Sinica

基　　金：安徽省自然科学基金项目(1908085MC70);安徽省教育厅优秀青年人才支持计划(gxyq2021167);中国烟草总公司四川省公司科技项目(SCYC202112);国家级大学生创新创业训练计划(202210364005)。

摘　　要：【目的】筛选对暗黑鳃金龟Holotrichia parallela幼虫具高毒力的球孢白僵菌Beauveria bassiana菌株,并评价最高毒力菌株分别与印楝素和辛硫磷联合使用的模拟田间防效。【方法】采用浸虫法,通过试虫校正死亡率和致死中时(medium lethal time,LT 50)值评价10株球孢白僵菌对暗黑鳃金龟3龄幼虫的毒力,并测定筛选出的Bb14菌株对暗黑鳃金龟1龄和3龄幼虫的致死中浓度(medium lethal concentration,LC 50)值。将Bb14菌株孢子悬浮液(1×10^(8)孢子/mL)分别与印楝素(3 mg/L)和辛硫磷(200 mg/L)联用,通过温室盆栽实验评价其对烟草幼苗上暗黑鳃金龟3龄幼虫的防治效果。【结果】在1×10^(8)孢子/mL处理下,供试10株球孢白僵菌以Bb14菌株对暗黑鳃金龟3龄幼虫毒力最高,其导致的17 d累计校正死亡率为76.67%,LT 50值为11.14 d。该菌株对暗黑鳃金龟1龄和3龄幼虫的LC 50值分别为0.32×10^(7)和0.68×10^(7)孢子/mL。盆栽实验中药后25 d时,单独使用Bb14菌株、印楝素和辛硫磷对暗黑鳃金龟3龄幼虫的防效分别为42.86%,64.29%和82.14%,而当Bb14分别与印楝素和辛硫磷联用后,对暗黑鳃金龟3龄幼虫的防效分别达到78.57%和92.86%。【结论】供试10株球孢白僵菌中Bb14菌株对暗黑鳃金龟幼虫的毒力最高,该菌株与印楝素或辛硫磷联用能够提高对暗黑鳃金龟幼虫的防效。【Aim】To screen Beauveria bassiana strains with high virulence against Holotrichia parallela larvae and to evaluate the simulated field efficacy of the strain with the highest virulence in combination with azadirachtin and phoxim,respectively.【Methods】The virulence of 10 strains of B.bassiana to the 3rd instar larvae of H.parallela was evaluated based on the corrected mortality and medium lethal time(LT 50)value using the insect dipping method.Furthermore,the medium lethal concentration(LC 50)values of the screened Bb14 strain against the 1st and 3rd instar larvae of H.parallela were determined.In the pot experiments,the conidial suspension of Bb14(1×10^(8)conidia/mL)was applied in combination with azadirachtin(3 mg/L)and phoxim(200 mg/L),respectively,and the control efficacy against the 3rd instar larvae of H.parallela on tobacco seedlings was evaluated in greenhouse.【Results】Among the 10 strains of B.bassiana at the concentration of 1.0×10^(8)conidia/mL,Bb14 had the highest virulence against the 3rd instar larvae of H.parallela,causing the cumulative corrected mortality of 76.67%in 17 d and with the LT 50 value of 11.14 d.The LC 50 values of Bb14 infecting the 1st and 3rd instar larvae of H.parallela were 0.32×10^(7)and 0.68×10^(7)conidia/mL,respectively.In the pot experiments,the control efficacy of Bb14,azadirachtin and phoxim against the 3rd instar larvae of H.parallela was 42.86%,64.29%and 82.14%,respectively,at 25 d after application.At 25 d after applying Bb14 in combination with azadirachtin and phoxim,respectively,the control efficacy against the 3rd instar larvae of H.parallela reached 78.57%and 92.86%,respectively.【Conclusion】Among the tested 10 strains of B.bassiana,Bb14 has the highest virulence to H.parallela larvae.The application of Bb14 in combination with azadirachtin and phoxim leads to improved control efficacy against H.parallela larvae.

关 键 词：暗黑鳃金龟 球孢白僵菌 毒力测定 田间防效评价 生物防治 

分 类 号：Q965.8[生物学—昆虫学]