不同家蚕品种五龄幼虫期与蛹期中肠细菌组成与功能差异分析  

Analysis of differences in the midgut bacterial composition and function between different Bombyx mori varieties at the 5th instar larval and pupal stages

作　　者：李庆荣[1,2] 杨琼 邢东旭[1,2] 邹宇晓 张伟龙[1] 肖阳 LI Qing-Rong;YANG Qiong;XING Dong-Xu;ZOU Yu-Xiao;ZHANG Wei-Long;XIAO Yang(Sericultural&Agri-Food Research Institute,Guangdong Academy of Agricultural Sciences,Guangzhou 510610,China;Key Laboratory of Urban Agriculture in South China,Ministry of Agriculture and Rural Affairs,Guangzhou 510610,China)

机构地区：[1]广东省农业科学院蚕业与农产品加工研究所,广州510610 [2]农业农村部华南都市农业重点实验室,广州510610

出　　处：《昆虫学报》2023年第8期1020-1030,共11页Acta Entomologica Sinica

基　　金：广东省农业科学院协同创新中心项目(XT202221);国家蚕桑产业技术体系项目(CARS-18-0503);广东省农业科学院农业优势产业学科团队建设项目(202119TD);广东省蚕桑产业技术体系蚕病防治岗位(2022KJ124);广东省乡村振兴战略专项资金种业振兴项目(2022-XBH-00-001,2022-XPY-00-014)。

摘　　要：【目的】分析生命力及茧层率不同的家蚕Bombyx mori品种间中肠细菌组成的差异及其对生命力与蚕茧量等性状的影响。【方法】基于长期的家蚕资源饲养调查成绩,选取了高生命力家蚕品种932G和高丝量品种2041J作为实验材料,收集5龄幼虫和蛹的中肠,采用高通量测序平台对中肠细菌16S rDNA进行测序分析,比较不同品种及不同发育阶段家蚕中肠细菌组成及其功能的差异。【结果】分别获得932G和2041J 5龄幼虫期中肠细菌399和453个可操作分类单元(operational taxonomic unit,OTU),蛹期OTU数目分别为138和162个。5龄幼虫期中肠优势菌门是变形菌门(Proteobacteria)、厚壁菌门(Firmicutes)及放线菌门(Actinobacteriota),优势属是甲基杆菌属Methylobacterium,丰度最高,其次为葡萄球菌属Staphylococcus。有多个菌属的相对丰度在932G和2041J间差异显著,其中德沃斯菌属Devosia、罗尔斯通菌属Ralstonia、硝化螺旋菌属Nitrospira、短杆菌属Brachybacterium、罗氏菌属Rothia、葡萄球菌属及劳氏菌属Lawsonella等在932G 5龄幼虫中肠中的丰度显著高于在2041J 5龄幼虫中肠中的,而假单胞菌属Pseudomonas、甲基杆菌属、不动杆菌属Acinetobscter、管道杆菌属Cloacibacterium、明串珠菌属Leuconostoc、丙酸杆菌属Propionibacteriaceae、嗜冷杆菌属Psychrobacter、鞘氨醇菌属Sphingobium和拟杆菌属Bacteroides等在2041J 5龄幼虫中肠中的相对丰度显著高于932G 5龄幼虫中肠中的。932G和2041J 5龄幼虫中肠细菌分别有77%和78%的功能基因富集在KEGG代谢通路上,其次是富集在环境信息处理和遗传信息处理通路的功能基因;932G的5龄幼虫中肠中硝酸盐还原、氮气呼吸、硝酸盐呼吸、亚硝酸盐呼吸及固氮等功能菌群占比高于2041J 5龄幼虫中肠中的,2041J 5龄幼虫中肠中化学异养、尿素分解、甲醇氧化等功能菌群占比高于932G 5龄幼虫中肠中的。5龄幼虫中肠细菌组成与蛹的显著�【Aim】To analyze the differences in the midgut bacterial composition among different varieties of Bombyx mori with different vitalities and cocoon shell ratios,and their effects on the related traits such as vitality and cocoon shell ratio.【Methods】Based on the results of the long-term feeding survey on B.mori resource,the variety 932G with high vitality and the variety 2041J with high silk yield were selected as experimental materials,the midguts of the 5th instar larva and pupa were collected,and the 16S rDNA sequences of the midgut bacteria were sequenced and analyzed by the high-throughput sequencing platform to compare the differences in the composition and function of the midgut bacteria between different varieties and different developmental stages of B.mori.【Results】A total of 399 and 453 operational taxonomic units(OTUs)of the midgut bacteria were obtained from 932G and 2041J at the 5th instar larval stage,and 138 and 162 OTUs of the midgut bacteria were obtained from 932G and 2041J at the pupal stage,respectively.The dominant phyla of the midgut bacteria of B.mori at the 5th instar larval stage were Proteobacteria,Firmicutes and Actinobacteriota,and the dominant genus was Methylobacterium with the highest abundance,followed by Staphylococcus.However,there were significant differences in the relative abundance of some genera between the two varieties.The relative abundance of some genera including Devosia,Ralstonia,Nitrospira,Brachybacterium,Rothia,Lawsonella etc.in the midgut of the 5th instar larva of 932G was significantly higher than that in the midgut of the 5th instar larva of 2041J.The relative abundance of Pseudomonas,Methylobacterium,Acinetobscter,Cloacibacterium,Leuconostoc,Propionibacteriaceae,Psychrobacter,Sphingobium,Bacteroides etc.in the midgut of the 5th instar larva of 2041J was significantly higher than that in the midgut of the 5th instar larva of 932G.There were 77%and 78%functional genes in the midgut bacteria of the 5th instar larvae of 932G and 2041J enriched on the meta

关 键 词：家蚕 品种 高通量测序 肠道微生物 5龄幼虫期 蛹期 

分 类 号：Q965[生物学—昆虫学]