Exceptionally high genetic variance of the doubled haploid(DH)population of poplar  

作　　者：Caixia Liu Sui Wang Yi Liu Meng Wang Erqin Fan Chen Liu Shikai Zhang Chuanping Yang Junhui Wang Heike W.Sederoff Xiangling You Vincent L.Chiang Su Chen Ronald R.Sederoff Guanzheng Qu 

机构地区：[1]College of Life Science,Northeast Forestry University,Harbin 150040,People’s Republic of China [2]State Key Laboratory of Tree Genetics and Breeding,Northeast Forestry University,Harbin 150040,People’s Republic of China [3]Key Laboratory of Soybean Biology in Chinese Ministry of Education,Northeast Agricultural University,Harbin 150030,People’s Republic of China [4]State Key Laboratory of Tree Genetics and Breeding,Key Laboratory of Tree Breeding and Cultivation of State Forestry Administration,Research Institute of Forestry,Chinese Academy of Forestry,Beijing 100091,People’s Republic of China [5]Department of Plant and Microbial Biology,North Carolina State University,Raleigh,NC 27695,USA [6]Forest Biotechnology Group,Department of Forestry and Environmental Resources,North Carolina State University,Raleigh,NC 27695,USA

出　　处：《Journal of Forestry Research》2023年第6期1941-1950,共10页林业研究（英文版）

基　　金：supported by the National Key R&D Program of China(2021YFD2200203);Heilongjiang Province Key R&D Program of China(GA21B010);Heilongjiang Touyan Innovation Team Program(Tree Genetics and Breeding Innovation Team);Heilongjiang Postdoctoral Financial Assistance(LBH-Z21097)。

摘　　要：Doubled haploid(DH)plants have been widely used for breeding and biological research in crops.Pop ulus spp.have been used as model woody plant species for biological research.However,the induction of DH poplar plants is onerous,and limited biological or breeding work has been carried out on DH individuals or populations.In this study,we provide an effective protocol for poplar haploid induction based on an anther culture method.A total of 96 whole DH plant lines were obtained using an F1hybrid of Populus simonii×P.nigra as a donor tree.The phenotypes of the DH population showed exceptionally high variance when compared to those of half-sib progeny of the donor tree.Each DH line displayed distinct features compared to those of the other DH lines or the donor tree.Additionally,some excellent homozygous lines have the potential to be model plants in genetic and breeding studies.

关 键 词：POPULUS Anther culture Doubled haploid Genetic variance Flow cytometry 

分 类 号：S792.11[农业科学—林木遗传育种]