一测多评法同时测定野生和家种防风药材中4种有效成分的含量  

Simultaneous Determination of Four Active Components in Wild and Cultivated Saposhnikovia Divaricata by One Test and Multiple Evaluation Method

作　　者：丘娴 李振雨 周湘媛 林碧珊[3] 马懿飞 何民友 赵书运 魏梅 孙冬梅 陈向东 Qiu Xian;Li Zhenyu;Zhou Xiangyuan;Lin Bishan;Ma Yifei;He Minyou;Zhao Shuyun;Wei Mei;Sun Dongmei;Chen Xiangdong(Guangdong Yifang Pharmaceutical Co.,Ltd.,Foshan 528244,China;Guangdong Provincial Key Laboratory of Traditional Chinese Medicine Formula,Foshan 528244,China;Guangdong Global Pharmaceutical Co.,Ltd.,Foshan 528000,China;China Traditional Chinese Medicine Holdings Co.,Ltd.,Foshan 528303,China)

机构地区：[1]广东一方制药有限公司,广东佛山528244 [2]广东省中药配方颗粒企业重点实验室,广东佛山528244 [3]广东环球制药有限公司,广东佛山528000 [4]中国中药控股有限公司,广东佛山528303

出　　处：《亚太传统医药》2023年第9期59-65,共7页Asia-Pacific Traditional Medicine

基　　金：佛山市核心技术攻关项目(1920001000378);广东省基础与应用基础研究基金项目(2020B1515120033)。

摘　　要：目的:采用一测多评法同时测定防风药材中升麻素苷、升麻素、5-O-甲基维斯阿米醇苷和亥茅酚苷的含量,为野生和家种防风药材质量评价提供依据。方法:色谱柱为Waters BEH C18(2.1 mm×100 mm,1.7μm);流动相为乙腈-水,梯度洗脱;流速为0.35 mL/min;检测波长为254 nm;柱温为30℃;进样量为1μL。以升麻素苷为内参物,测定升麻素、5-O-甲基维斯阿米醇苷和亥茅酚苷的相对校正因子,利用相对校正因子计算含量,建立一测多评法,并将一测多评的计算结果与外标法测定结果进行比较,从而确定一测多评的准确性。同时比较野生和家种防风4种有效成分的含量差异。结果:采用所建立的一测多评法计算的防风药材升麻素、5-O-甲基维斯阿米醇苷和亥茅酚苷的含量与外标法计算的含量相对偏差均小于5.0%,野生防风中升麻素、5-O-甲基维斯阿米醇苷和亥茅酚苷含量均明显高于家种防风(P<0.05),而升麻素苷在野生与家种中的含量无明显差异(P>0.05)。结论:该一测多评法准确、便捷,能够为防风药材的质量评价提供参考依据。Objective:To provide the basic for the quality evaluation of wild and cultivated Saposhnikovia divaricata,the contents of cimicifugoside,cimifugin,5-O-methylvisamitol glycoside and sec-O-glucosylhamaudol were simultaneously determined by the method of quantitative analysis of multi-components by single marker(QAMS).Methods:The determination was performed on Waters BEH C_(18)(2.1mm×100mm,1.7μm)column.The mobile phase consisted of acetonitrile-water with gradient elution.The flow rate was 0.35mL/min.The detection wavelength was 254nm.The column temperature was 30℃.The injection volume was 1μL.The cimicifugoside was used as internal reference to determine the relative correction factors of cimifugin,5-O-methylvisamitol glycoside and sec-O-glucosylhamaudol.The content was calculated by relative calibration factors to develop a method of quantitative analysis of multi-components.Comparing the results of QAMS with those of external standard method(ESM),the accuracy of one-evaluation and multi-evaluation was be determined.At the same time,the contents of four effective components of wild and cultivated Saposhnikovia divaricata were compared.Results:The average relative deviations between the results of QAMS and ESM were less than 5.0%.The contents of cimifugin,5-O-methylvisamitol glycoside and sec-O-glucosylhamaudol in wild Saposhnikovia divaricata were significantly higher than those in cultivated Saposhnikovia divaricata(P<0.05).There was no significant difference in the content of cimicifugoside between the wild and cultivated species.Conclusion:The QAMS method was accurate and convenient,which provided reference for the quality evaluation of Saposhnikovia divaricata.

关 键 词：防风 一测多评 野生 家种 质量评价 

分 类 号：R284.2[医药卫生—中药学]