新疆拜城县马铃薯疮痂病病原菌的分离鉴定及生物学特性分析  

Isolation, identification and biological characteristicsof potato scab pathogen in Baicheng, Xinjiang

作　　者：徐李娟 陈勇[3] 王则玉[3] 王博[2] 艾尼江·尔斯满 郭瑞 李克梅[1] 宋素琴[2,4] XU Lijuan;CHEN Yong;WANG Zeyu;WANG Bo;Ainijiang Ersiman;GUO Rui;LI Kemei;SONG Suqin(College of Agriculture,Xinjiang Agricultural University,Urumqi 830052,China;Institute of Microbiology Application,Xinjiang Academy of Agricultural Sciences/Xinjiang key Laboratory of Special Environmental Microorganisms,Urumqi 830091,China;Institute of soil,Fertilizer and Agricultural Water Conservation,Xinjiang Academy of Agricultural Aciences,Urumqi 830091,China;College of Agriculture,Shihezi University/Key Laboratory at the Universities of Xinjiang Uygur Autonomous Region for Oasis Agricultural Pest Management and Plant Protection Resource Utilization,Shihezi Xinjiang 832003,China)

机构地区：[1]新疆农业大学农学院,乌鲁木齐830052 [2]新疆农业科学院微生物应用研究所/新疆特殊环境微生物重点实验室,乌鲁木齐830091 [3]新疆农业科学院土壤肥料与农业节水研究所,乌鲁木齐830091 [4]新疆绿洲农业病虫害治理与植保资源利用自治区高校重点实验室/石河子大学农学院,新疆石河子832003

出　　处：《新疆农业科学》2023年第9期2258-2265,共8页Xinjiang Agricultural Sciences

基　　金：新疆维吾尔自治区重点研发项目任务专项“新疆马铃薯绿色高产高效栽培技术研究与集成应用”(2022B02044-2);新疆农业科学院科技创新重点培育专项“马铃薯疮痂病病原菌致病毒素thaxtomins产量差异分析及其对新疆农田杂草的除草性能研究”(xjkcpy-202203);农业农村部西北荒漠绿洲作物有害生物综合治理重点实验室开放基金课题“新疆马铃薯疮痂病病原菌Steptomyces spp.多样性及其致病力分化研究”(KFJJ202006)。

摘　　要：【目的】研究新疆阿克苏地区拜城县马铃薯疮痂病病原菌的种类。【方法】采集新疆阿克苏地区拜城县3个不同种植区的马铃薯疮痂病块茎及薯表土壤,采用组织分离法和稀释涂布分离法,并通过致病性测定、形态学、分子生物学及生理生化特性对病原菌进行鉴定。【结果】在分离出的72株放线菌中,菌株K6含致病基因txtAB、tomA、nec1且对萝卜幼苗有较强致病性;经鉴定为同一种链霉菌属Streptomyces bobili,为新疆首次报道的病原菌。所有供试碳源、氮源均可作为单一碳源、氮源被菌株S.bobili K6利用,在酪氨酸琼脂培养基上有黑色素产生,对6%NaCl、0.1%苯酚、20μg/mL链霉素敏感,在pH4的条件下不能正常生长。【结论】新疆拜城县马铃薯疮痂病病原菌为S.bobili,包含3种致病基因txtAB、tomA、nec1,且致病性较强。【Objective】To clarify the species of potato common scab pathogenic bacteria in Baicheng County,Aksu Prefecture,Xinjiang.【Methods】Potato common scab tubers and potato surface soil were collected from three different growing areas in Baicheng County,Aksu Prefecture,Xinjiang,and the pathogenic bacteria were identified by tissue isolation method and dilution coating isolation method,and by pathogenicity determination,morphology,molecular biology and physiological and biochemical characteristics.【Results】Among the 72 isolated actinomycetes,six strains contained the pathogenic genes txtAB,tomA and nec1 and were strongly pathogenic to radish seedlings;they were identified as the same Streptomyces bobili,which was the first reported pathogen in Xinjiang.All the tested carbon and nitrogen sources were available as single carbon and nitrogen sources by strain S.bobili K6,with melanin production on complex agar medium,sensitive to 6%NaCl,0.1%phenol,20μg/mL streptomycin,and not growing any more at pH 4 normally.【Conclusion】The pathogenic of potato common scab is S.bobili in Baicheng County of Xinjiang,which contains three pathogenic genes txtAB,tomA,and nec1,and belongs to highly pathogenicity.

关 键 词：马铃薯疮痂病病原菌 链霉菌 分离鉴定 生物学特性 

分 类 号：S436.32[农业科学—农业昆虫与害虫防治]