重金属镉胁迫对甜菜夜蛾体内保幼激素和蜕皮激素滴度的影响  

Effects of cadmium stress on juvenile hormone andmolting hormone titers in Spodoptera exigua

作　　者：吴梦露 孙清明[2] 吴娇娇 王凯 杨旸 杨益众[1] 苏宏华[1] WU Menglu;SUN Qingming;WU Jiaojiao;WANG Kai;YANG Yang;YANG Yizhong;SU Honghua(College of Horticulture and Plant Protection,Yangzhou University,Yangzhou Jiangsu 225009,China;Key Laboratory of Biology and Genetic resources Utilization of Subtropical Fruit Trees,Ministry of Agriculture and Rural Areas/Institute of Fruit Tree Research,Guangdong Academy of Agricultural Sciences,Guangzhou 510640,China)

机构地区：[1]扬州大学园艺与植物保护学院,江苏扬州225009 [2]广东省农业科学院果树研究所/农业农村部南亚热带果树生物学与遗传资源利用重点实验室,广州510640

出　　处：《新疆农业科学》2023年第9期2291-2297,共7页Xinjiang Agricultural Sciences

基　　金：农业农村部:南亚热带果树生物学与遗传资源利用重点实验室开放课题(212103)。

摘　　要：【目的】研究重金属镉胁迫对昆虫生长发育的影响,为重金属镉胁迫评价提供依据。【方法】采用镉含量分别为0、0.2、3.2和51.2 mg/kg的人工饲料饲喂甜菜夜蛾初孵幼虫直至化蛹,测定不同虫态(3龄幼虫、5龄幼虫、雌/雄成虫)甜菜夜蛾体内保幼激素和蜕皮激素的滴度。【结果】低浓度镉(0.2 mg/kg)胁迫下甜菜夜蛾幼虫体内保幼激素和蜕皮激素滴度升高;随着镉浓度的升高,保幼激素和蜕皮激素的滴度逐渐下降。镉胁迫下甜菜夜蛾成虫体内保幼激素滴度升高,蜕皮激素滴度降低;随着镉浓度的升高,保幼激素(JH)和蜕皮激素(MH)的滴度无明显变化。【结论】JH和MH是昆虫生长发育和繁殖的重要调控因子,重金属镉胁迫通过改变昆虫体内JH和MH的滴度进而对昆虫生长发育和生殖产生影响。【Objective】Effects of cadmium stress on juvenile hormone and molting hormone titers in Spodoptera exigua.The effect of heavy metal on growth and development of insects from the perspective of insect physiology in the hope of providing a basis for ecological risk assessment and pollution control of heavy metals.【Methods】In this study,artificial diet containing four concentrations of Cd 2+(0,0.2,3.2,51.2 mg/kg)were used to feed the newly hatched larvae of Spodoptera exigua until pupation.Then the titers of juvenile hormone and molting hormone at different growth stages(3rd instar,5th instar,female adult and male adult)of S.exigua were determined.【Results】The results of this experiment showed that the titers of juvenile hormone and molting hormone in larvae increased under low concentration cadmium stress(0.2 mg/kg).With the increase of cadmium concentration,the titers of juvenile hormone and molting hormone gradually decreased.The titers of juvenile-preserving hormone and molting hormone increased in Spodoptera exigua under cadmium stress,and the titers of juvenile-preserving hormone and molting hormone did not change significantly with the increase of cadmium concentration.【Conclusion】The results indicate that cadmium stress has significant effects on the titers of two hormones in S.exigua.

关 键 词：金属镉 甜菜夜蛾 保幼激素 蜕皮激素 

分 类 号：S435.663[农业科学—农业昆虫与害虫防治]