苦蒿素改善新生大鼠急性肺损伤的作用机制  

Mechanism of Blinin on Improving Acute Lung Injury in Neonatal Rats

作　　者：杨亚龙 林文东 林清凡 YANG Yalong;LIN Wendong;LIN Qingfan(Quanzhou Medical College,Quanzhou,Fujian,362100,China)

机构地区：[1]泉州医学高等专科学校,福建省泉州市362100

出　　处：《医学分子生物学杂志》2023年第5期439-443,共5页Journal of Medical Molecular Biology

基　　金：泉州市科技计划项目(No.2020N058s)。

摘　　要：目的分析苦蒿素改善急性肺损伤(acute lung injury,ALI)的机制。方法60只新生大鼠随机等分为假手术组、模型组、苦蒿素低、中、高剂量组和地塞米松组。血氧分压(PaO_(2))和肺干湿重比(W/D)评估肺水肿;HE染色检测肺组织病理损伤;ELISA检测大鼠支气管肺泡灌洗液(bronchoalveolar lavage fluid,BALF)中氧化应激和炎性水平;蛋白质印迹法检测大鼠肺组织高迁移率族蛋白B1(high mobility group box 1 protein,HMGB1)/核因子κB(nuclear factor kappa-B,NF-κB)信号通路相关蛋白表达。结果与模型组相比,苦蒿素高剂量组PaO_(2)明显上升,W/D和肺损伤评分、BALF炎症和氧化应激水平、肺组织HMGB1、TLR4和p-NF-κB P65/NF-κB P65表达明显下降(P<0.05)。结论苦蒿素通过提高抗氧化抗炎活性及阻断HMGB1/NF-κB信号通路改善ALI。Objective To analyze the mechanism of blinin in improving acute lung injury(ALI).Methods A total of 60 neonatal rats were randomly divided into 6 groups,the sham operation group,the model group,the blinin low-,medium-and high-dose groups,and the dexamethasone group.The pulmonary edema was assessed by the blood partial pressure of oxygen(PaO_(2))and the lung wet/dry weight ratio(W/D).The pathological damage of lung tissues was detected by HE staining.The levels of oxidative stress and inflammation factors in the bronchoalveolar lavage fluid(BALF)were detected by ELISA.The expression levels of the high mobility group box 1 protein(HMGB1)/nuclear factor kappa-B(NF-κB)related proteins in lung tissues were detected by Western blotting.Results The PaO_(2) was significantly increased in the blinin high-dose group when compared with that in the model group,while the W/D and lung injury scores,the levels of inflammation and oxidative stress fators in BALF,and the expression levels of HMGB1,TLR4 and p-NF-κB p65/NF-κB p65 in lung tissues were significantly decreased(P<0.05).Conclusion Blinin can improve ALI by enhancing anti-oxidation and anti-inflammatory activity and blocking the HMGB1/NF-κB signaling pathways.

关 键 词：苦蒿素 急性肺损伤 高迁移率族蛋白B1 TOLL受体4 核因子ΚB 氧化应激反应 

分 类 号：R563[医药卫生—呼吸系统]