抗-c、抗-E、抗-Jk^(a)和抗-Fy^(a)复杂联合抗体的鉴定  

Identification of Complex and Combined Antibody Consisted of Anti-c,Anti-E,Anti-Jk^(a)and Anti-Fy^(a)

作　　者：马婷婷 刘学军 黄宝甲 周燕 莫秋红 钟周琳 刘金莲 MA Ting-Ting;LIU Xue-Jun;HUANG Bao-Jia;ZHOU Yan;MO Qiu-Hong;ZHONG Zhou-Lin;LIU Jin-Lian(Nanning Institute of Transfusion Medicine,Nanning Blood Center,Nanning 530007,Guangxi Zhuang Autonomous Region,China)

机构地区：[1]南宁输血医学研究所,南宁中心血站,广西南宁530007

出　　处：《中国实验血液学杂志》2023年第5期1475-1480,共6页Journal of Experimental Hematology

基　　金：广西壮族自治区卫生健康委员会自筹经费科研课题(Z20201430)。

摘　　要：目的:探讨多种血型血清学方法的联合运用在复杂抗体鉴定中的作用。方法:采用盐水法和微柱凝集法检测患者的血型抗原,采用盐水法筛选及鉴定患者血清中的Ig M类抗体,采用凝聚胺法、抗球蛋白法、微柱凝集法、酶法和吸收放散法筛选及鉴定Ig G类抗体。结果:检出1例患者血型为B型/CCDee/Jk(a-b+)/Fy(a-b+)。患者血清与谱细胞反应,在盐水介质中呈抗-E格局;在微柱凝集卡中全阳性,但与经木瓜酶处理的谱细胞反应出现2个阴性。参照格局表考虑存在抗-c、E和抗-Jk^(a)抗体,以及1种易被木瓜酶破坏的血型抗原对应的抗体。选用特定表型的红细胞对患者血清进行吸收放散试验,检出Ig G类抗-c、抗-E、抗-Jk^(a)和抗-Fy^(a)抗体。结论:联合运用多种血型血清学方法证实该患者血清中存在Ig M类抗-E和Ig G类抗-c、抗-E、抗-Jk^(a)及抗-Fy^(a)联合抗体。Objective:To investigate the role of multiple serological methods in the identification of complex antibodies.Methods:The blood group antigens were detected by saline and microcolumn agglutination methods.The saline method was used to screen and identify IgM-type antibodies in the patient′s serum,while the polybrene,antiglobulin,microcolumn agglutination,enzymic and absorption-elution methods were used to screen and identify IgG-type antibodies.Results:The patient was B/CCDee/Jk(a-b+)/Fy(a-b+)blood type.The serum reacted with panel cells,and the reaction presented anti-E pattern in the saline medium.It was fully positive in the microcolumn agglutination card,except 2 negative ones after using papain to treat the panel cells.Referring to the pattern table,it was concluded that there existed anti-c,anti-E,and anti-Jk^(a)antibodies,and one antibody corresponding to an antigen that was easily destroyed by papain.The red blood cells with specific phenotype were selected for absorption-elution to identify IgG-type anti-c,anti-E,anti-Jk^(a)and anti-Fy^(a)antibodies.Conclusion:It is confirmed that IgM-type anti-E,and IgG-type anti-c,anti-E,antiJk^(a)and anti-Fy^(a)antibodies exist in the patient′s serum by multiple serological methods.

关 键 词：血型血清学方法 抗体鉴定 抗-C 抗-E 抗-Jk^(a) 抗-Fy^(a) 

分 类 号：R457.11[医药卫生—治疗学]